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Toxicological information

Basic toxicokinetics

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Administrative data

Endpoint:
basic toxicokinetics
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1988
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: The documentation is from secondary literature.
Justification for type of information:
The justification for read across is provided as an attachment in IUCLID Section 13.
Cross-reference
Reason / purpose:
read-across: supporting information
Reference
Endpoint:
basic toxicokinetics
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
supporting study
Study period:
1988
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: The documentation is from secondary literature.
Justification for type of information:
The justification for read across is provided as an attachment in IUCLID Section 13.
Reason / purpose:
read-across source
Executive summary:

This data is being read across from the source study that tested 2, 6, 10, 14-tetramethylpentadecane based on analogue read across.

The fate of pristane (2, 6, 10, 14-tetramethylpentadecane) was studied in rats after a single per os administration of 3H-labeled pristane. The balance study showed extensive fecal excretion (66%) mainly as unchanged hydrocarbon, whereas about 14% of ingested pristane was excreted in urine as pristane metabolites and tritiated water. After one week, 8.3% of the ingested 3H still was stored in the carcass and the radioactive distribution in tissues and organs showed a preferential incorporation into adipose tissue and liver. Over 75% of the radioactivity stored in the carcass was associated with pristane metabolites and tritiated water. Tissue metabolites were characterized by thin layer chromatography, gas chromatography, and mass spectrometric analyses. Four metabolites were identified: pristan-1-ol, pristane-2-ol, pristanic acid and 4, 8, 12-trimethyltridecanoic acid. These results demonstrated that pristane undergoes subterminal hydroxylation or terminal oxidation followed by the classical beta-oxidation process. Incorporation of metabolites in phospholipids and more particularly in the phosphatidylserine fraction has been observed.

Data source

Reference
Reference Type:
publication
Title:
Disposition and metabolism of pristane in rat.
Author:
Le Bon A M; Cravedi J P; Tulliez J E
Year:
1988
Bibliographic source:
Lipids, (1988 May) Vol. 23, No. 5, pp. 424-9. ISSN: 0024-4201.

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Mass Balance

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent

Results and discussion

Applicant's summary and conclusion

Executive summary:

The fate of pristane (2, 6, 10, 14-tetramethylpentadecane) was studied in rats after a single per os administration of 3H-labeled pristane. The balance study showed extensive fecal excretion (66%) mainly as unchanged hydrocarbon, whereas about 14% of ingested pristane was excreted in urine as pristane metabolites and tritiated water. After one week, 8.3% of the ingested 3H still was stored in the carcass and the radioactive distribution in tissues and organs showed a preferential incorporation into adipose tissue and liver. Over 75% of the radioactivity stored in the carcass was associated with pristane metabolites and tritiated water. Tissue metabolites were characterized by thin layer chromatography, gas chromatography, and mass spectrometric analyses. Four metabolites were identified: pristan-1-ol, pristane-2-ol, pristanic acid and 4, 8, 12-trimethyltridecanoic acid. These results demonstrated that pristane undergoes subterminal hydroxylation or terminal oxidation followed by the classical beta-oxidation process. Incorporation of metabolites in phospholipids and more particularly in the phosphatidylserine fraction has been observed.