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Acute tests on all three trophic levels were performed to examine the aquatic toxicity of vinylcaprolactame. Algae and daphnids turned out to be the most sensitive species revealing an E(r)C50 (48h/72h) >100 mg/L, respectively. Effects of the substance towards fish were found in the same range providing a LC50 (96h) of 318 mg/L. Thus, vinylcaprolactame is with high probability acutely not harmful to aquatic organisms.