Methyl chloroacetate

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: No guideline was followed and no GLP. However, the test method was similar to OECD 402.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Principles of method if other than guideline:

No guideline was followed but the test method was similar to OECD 402.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Methyl chloroacetate

EC Number:

202-501-1

EC Name:

Methyl chloroacetate

Cas Number:

96-34-4

Molecular formula:

C3H5ClO2

IUPAC Name:

methyl 2-chloroacetate

Details on test material:

- Name of test material (as cited in study report): monochloressigsäuremethylester
- Physical state: colourless liquid

Test animals

Species:

rat

Strain:

Wistar

Sex:

female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: own breeding
- Weight at study initiation: 199 ± 9.93 g
- Diet (e.g. ad libitum): Altromin 1324 (Altromin GmbH, Lage/Lippe), ad libitum
- Water (e.g. ad libitum): tap water, ad libitum

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

TEST SITE
- Area of exposure: ca. 30 cm2
- Type of wrap if used: aluminium foil (6 x 8 cm) wrapped by an elastic plaster (Elastoplast, 8 cm wide)


REMOVAL OF TEST SUBSTANCE
- Washing (if done): with water
- Time after start of exposure: 24 hours

Duration of exposure:

24 hours

Doses:

50, 100, 125, 160, 315 and 500 mg/kg bw

No. of animals per sex per dose:

Six females per dose

Control animals:

not required

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: body weight was recorded at 0, 7 and 14 days
- Other examinations performed: clinical signs, necropsy.

Statistics:

Probit analysis

Results and discussion

Effect levels

Sex:

female

Dose descriptor:

LD50

Effect level:

136.6 mg/kg bw

95% CL:

121.3 - 153.7

Mortality:

Mortality was observed at 125, 160, 315 and 500 mg/kg bw.

Clinical signs:

other: Prone position, passivity, irregular and laboured respiration, squatting position, piloerection, dizziness, impared balance, hyporeflexia and increased red coloured lacrimation.

Gross pathology:

Animals showed clearly extended blood vessels in the subcutaneous area of the treated skin.

Any other information on results incl. tables

Table 7.2.3 -1: Mortality

Dose (mg/kg bw)	Mortality
50	0/6
100	0/6
125	2/6
160	5/6
315	6/6
500	6/6

Applicant's summary and conclusion

Interpretation of results:

Toxicity Category II

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The dermal LD50 was 136.6 mg/kg bw with a 95% confidence interval between 121.3 and 153.7 mg/kg bw.

Executive summary:

The test substance methyl chloroacetate was administered dermally to groups of female Wistar rats at the doses of 50, 100, 125, 160, 315 and 500 mg/kg bw during 24 hours. Animals were subjected to daily observations and weekly determinations of body weight. Macroscopic examination was performed in the animals. Mortality was observed at 125, 160, 315 and 500 mg/kg bw. The dermal LD50 was 136.6 mg/kg bw with a 95% confidence interval between 121.3 and 153.7 mg/kg bw.