Methyl chloroacetate

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: OECD guideline and GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method B.2 (Acute Toxicity (Inhalation))

Deviations:

no

GLP compliance:

yes

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Methyl chloroacetate

EC Number:

202-501-1

EC Name:

Methyl chloroacetate

Cas Number:

96-34-4

Molecular formula:

C3H5ClO2

IUPAC Name:

methyl 2-chloroacetate

Details on test material:

- Name of test material (as cited in study report): monochloressigsäuremethylester
- Analytical purity: 99%

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Hoechst AG, Kastengrund, SPF-Zucht
- Age at study initiation: 8-10 months
- Weight at study initiation: males: 203-225 g; females: 191-220 g
- Diet (e.g. ad libitum): Altromin 1324 (Altromin-GmbH, Lage/Lippe), ad libitum
- Water (e.g. ad libitum): tap water, ad libitum


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 2 ºC
- Humidity (%): 50 ± 20%
- Photoperiod: 12 hours light/12 hours dark

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other: unchanged (no vehicle)

Details on inhalation exposure:

The test substance was applied continuously with a permanent drip apparatus and was evaporated at 130 ºC.

Analytical verification of test atmosphere concentrations:

yes

Duration of exposure:

4 h

Concentrations:

90, 210, 315 and 385 ppm

No. of animals per sex per dose:

5 animals per sex per dose

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: two daily observations and body weights were recorded at 0, 7 and 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, necropsy.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

210 - 315 ppm

Exp. duration:

4 h

Mortality:

Mortality was observed at 315 and 385 ppm.

Clinical signs:

other: 90 ppm: squatting position, uncoordinated walk, irregular and laboured respiration. At the higher dose groups: prone position, reeling walk, noisy respiration, hyporeflexia, corneal opacity and cyanose.

Gross pathology:

Dark red dyed lungs. While cutting the lung, foamy liquid escaped in the majority of the animals. The intestine mucous membrane was red and was filled with foamy liquid.

Any other information on results incl. tables

Table 7.2.2 -2: Mortality

Concentration (ppm)	Mortality
	Males	Females
90	0/5	0/5
210	0/5	0/5
315	3/5	4/5
385	5/5	5/5

Applicant's summary and conclusion

Interpretation of results:

Toxicity Category II

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The 4-h LC50 in rats was determined to be between 210 and 315 ppm (638 and 957 mg/m3).

Executive summary:

An acute inhalation toxicity study was carried out with the substance methyl chloroacetate. Groups of five Wistar rats per sex were exposed at 90, 210, 315 and 385 ppm for 4 hours. The 4-h LC50 in rats was determined to be between 210 and 315 ppm (638 and 957 mg/m3).