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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2003
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Version / remarks:
and Directive CEE - Method C3
GLP compliance:
yes (incl. QA statement)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Study design

Limit test:
yes
Total exposure duration:
72 h
Remarks on exposure duration:
observations of cell growth at 24, 48 and 72h

Test conditions

Hardness:
LC Reconstituted water; hardness of 34 +/- 17 mg CaCO3/L.
Test temperature:
22.7 to 23.2°C
pH:
7.34 to 8.06
Nominal and measured concentrations:
See table below in the window: Any other information on the method incl. figures and tables.
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
other: ErC50 (algae specific grow th rate)
Effect conc.:
> 37.1 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks:
Specific growth rate: natural log of the cell number over specific intervals (0-24 h, 0-48 h, 0-72 h)
Duration:
48 h
Dose descriptor:
other: ErC50 (algae specific groth rate)
Effect conc.:
> 35.7 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks:
Specific growth rate: natural log of the cell number over specific intervals (0-24 h, 0-48 h, 0-72 h)
Duration:
72 h
Dose descriptor:
other: ErC50 (algae specific growth rate)
Effect conc.:
> 34.2 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks:
Specific growth rate: natural log of the cell number over specific intervals (0-24 h, 0-48 h, 0-72 h)
Duration:
72 h
Dose descriptor:
other: EbC50
Effect conc.:
> 34.2 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks:
(cell.ml-1.h: increase in cell number/ml of solution x time (h)
Results with reference substance (positive control):
None.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
No Observed Effect Concentration (NOEC) at 72h: >= 34.2mg/L, based on specific growth rate and biomass data.
Not classified.