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Diss Factsheets
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EC number: 203-813-0 | CAS number: 110-89-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Specific investigations: other studies
Administrative data
- Endpoint:
- biochemical or cellular interactions
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- publication
- Title:
- BIOCHEMICAL STUDIES OF SIX NITROGEN-CONTAINING HETEROCYCLES IN RAT TISSUES
- Author:
- Kitchin, K.T. et al.:
- Year:
- 1 989
- Bibliographic source:
- Biochem. Pharmacol. 38, 2733-2738
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Rats were treated orally with the test compound at 21 and 4 hours before they were killed. Biochemical examinations were performed in different tissues and blood.
- GLP compliance:
- not specified
- Type of method:
- in vivo
- Endpoint addressed:
- basic toxicokinetics
Test material
- Reference substance name:
- Piperidine
- EC Number:
- 203-813-0
- EC Name:
- Piperidine
- Cas Number:
- 110-89-4
- Molecular formula:
- C5H11N
- IUPAC Name:
- piperidine
Constituent 1
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): piperidine
- Analytical purity: 98 %
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Laboratories (Wilmington, MA)
- Age at study initiation: 90 days
- Housing: 3 per cage
ENVIRONMENTAL CONDITIONS
- no data
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- physiological saline
- Details on exposure:
- PREPARATION OF DOSING SOLUTIONS:
- Piperidine was diluted with 0.9 % saline solution. Solution was adjusted to pH 7.0 before use. - Analytical verification of doses or concentrations:
- no
- Duration of treatment / exposure:
- Single treatment 21 and 4 hours before killing.
- Frequency of treatment:
- once
- Post exposure period:
- 21 and 4 hours
Doses / concentrations
- Dose / conc.:
- 80 mg/kg bw/day (actual dose received)
- Remarks:
- corresponding to 1/5 of the published LD50
- No. of animals per sex per dose:
- Piperidine: 7 animals; control: 8 animals
- Control animals:
- yes, concurrent vehicle
- Details on study design:
- - Leukocytes were separated from erythrocytes;
- Subcellular fractions were prepared from liver and esophagus:
• liver tissue was homogenized and used for alkaline elution.
• esophagus tissue digestion and lysis (on the filter) was performed to reduce DNA damage observed with homogenization.
- alkaline elution procedure was performed according to Kohn KW et al., 1981 (DNA Repair, A Laboratory Manual of Research Procedures (Eds. Hanawalt PC and Friedberg EC), pp 379-401. Marcel Dekker, New York).
- Assays for ODC activity, glutathione content, cytochrome P-450 content and SGPT activity were performed by standard methods.
- Statistical analysis of the data was done with analysis of variance. Statistically significant differences found by a Tukey's test were then further evaluated with a Student's t-test.
Examinations
- Examinations:
- - hepatic DNA damage
- ornithine decarboxylase (ODC) activity
- serum alanine aminotransferase (SGPT) activity
- cytochrome P-450
- glutathione content
Results and discussion
- Details on results:
- - Prior to sacrifice no animals died and no obvious clinical signs of toxicity were visually noted.
- Piperidine (80 mg/ kg bw) did not alter any of the six biochemical parameters:
• Blood alkaline elution (fraction of DNA eluted): Control: 0.070 ± 0.021; Piperidine 0.049 ± 0.011
• Liver alkaline elution (fraction of DNA eluted): Control: 0.100 ± 0.010; Piperidine 0.101 ± 0.009
• Liver ornithine decarboxylase (nmol CO2/g liver / hr): Control: 1.44 ± 0.32; Piperidine 3.13 ± 0.82
• Liver glutathione (µmol/g): Control: 3.94 ± 0.39; Piperidine 3.34 ± 0.33
• Liver cytochrome P-450 (nmol/g): Control: 3.52 ± 0.36; Piperidine 4.13 ± 0.33
• Serum alanine aminotransferase (I.U./I.): Control: 11.1 ± 0.8; Piperidine 12.3 ± 2.1 (this parameter was determined in 6 animals treated with piperidine).
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.