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EC number: 203-924-4 | CAS number: 111-96-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / bone marrow chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- received: 26 Nov 1982
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study performed similar to current OECD guideline with some deviations.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 983
- Report date:
- 1983
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 475 (Mammalian Bone Marrow Chromosome Aberration Test)
- Deviations:
- yes
- Remarks:
- 2 dose levels tested, no information on mitotic index
- GLP compliance:
- not specified
- Type of assay:
- mammalian bone marrow chromosome aberration test
Test material
- Reference substance name:
- Bis(2-methoxyethyl) ether
- EC Number:
- 203-924-4
- EC Name:
- Bis(2-methoxyethyl) ether
- Cas Number:
- 111-96-6
- Molecular formula:
- C6H14O3
- IUPAC Name:
- 1-methoxy-2-(2-methoxyethoxy)ethane
- Reference substance name:
- Diglyme
- IUPAC Name:
- Diglyme
- Reference substance name:
- Bis(2-methoxyethyl)ether
- IUPAC Name:
- Bis(2-methoxyethyl)ether
- Details on test material:
- Batch no. 21150
obtained from Aldrich Chemical Company, Wisc., USA
clear, colorless liquid
Constituent 1
Constituent 2
Constituent 3
Test animals
- Species:
- rat
- Strain:
- Crj: CD(SD)
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- obtained from Charles River (U.K.)
Administration / exposure
- Route of administration:
- inhalation: vapour
- Vehicle:
- Nitrogen
- Details on exposure:
- - Atmosphere generation and analysis in animal tests:
The test atmosphere was produced by bubbling dry, oxygen-free Nitrogen through Bis(2-methoxyethyl)ether contained in a Drechsel bottle immersed in a temperature controlled water bath at 50°C. The Nitrogen/vapor mixture so generated was diluted with filtered, conditioned compressed air and passed into the rat exposure chambers. These were 1.5 m3 capacity stainless steel and glas-chambers in which individually caged rats were confined to a single tier of cage occupying 0.5 m3. The chamber was ventilated at a rate of 12 air changes per hour.
Atmospheres in the chambers were analysed by infrared spectroscopy with Miran-1A Gas Analysers. Samples of chamber air were pumped continuously through the instruments and the chamber concentrations were automatically recorded. Instrument calibration was performed by a closed-loop calibration system. Known volumes of test compound were sequentially injected into the gas analyser with a Hamilton glass microsyringe. After each injection the absorbance reading was allowed to stabilise as indicated on the chart recording.
- Exposure procedure
Rodents were exposed to Diethylene glycol dimethyl ether atmospheres for 7 h/d for either 1 or 5 days. In the positive control groups EMS (please refer to "positive controls") was administered orally to all animals. Multiple dosed rodents received 100 mg/kg bw/d for 5 days while single-dosed rats received 250 mg/kg bw. - Duration of treatment / exposure:
- 7 h/d
- Frequency of treatment:
- (a) single exposure
(b) 5 consecutive days - Post exposure period:
- (a) 6, 24 or 48 h
(b) 6 h
Doses / concentrationsopen allclose all
- Dose / conc.:
- 250 ppm (analytical)
- Dose / conc.:
- 1 000 ppm (analytical)
- No. of animals per sex per dose:
- 10
- Control animals:
- yes, concurrent no treatment
- Positive control(s):
- Ethyl methanesulfonate (EMS) orally
Examinations
- Tissues and cell types examined:
- Cytogenetic analysis of rat bone marrow cells
- Details of tissue and slide preparation:
- Groups of 10 males and 10 females were exposed to the test item or filtered air either 1 or 5 days. After their last exposure period, animals were injected i.p. with 3 mg Colchicine/kg bw 2 h prior to death. The 1-day-exposed groups were killed at one of three sampling times namely, 6, 24, and 48 h after the end of exposure. The 5-day-exposed groups were killed 6 h after the end of exposure. Giemsa-stained slides were labeled with numbers not corelated with the animal numbers; therefore, all assessments of metaphases were "blind". Where possible, 50 metaphases per rat were scored.
- Evaluation criteria:
- no data
- Statistics:
- no data
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- no effects
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- - Clinical observations on rats
Male and female rat body weight was unaffected. All multiply exposed mammals in the 1000 ppm atmosphere groups were subdued and unresponsive to audio stimuli during exposure.
- Cytogenetic analysis of rat bone marrow sells
In neither the 5-days nor the single exposure test was there any evidence for the induction of chromosomal damage. the only significant increases in aberrant cell frequency occured in low-dose groups 6 h following a single exposure to the test material. In male rats exposed to 250 ppm Bis(2-methoxyethyl)ether, there was a small increase in the frequency of the total aberrations (p < 0.05). These elevations were restricted to a single sex in each case and were not reproduced at the higher dose levels. It was concluded, therefore, that Diethylene glycol dimethyl ether was not clastogen.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results: negative
Diethylene glycol dimethyl ether did not produce detectable significant aberrations of the bone marrow metaphase chromosomes of rats when inhaled at concentration up to 1000 ppm. - Executive summary:
Diethylene glycol dimethyl ether was tested in the chromosome aberration test in rats. Rodents were exposed to Diethylene glycol dimethyl ether atmospheres for 7 h/d for either 1 or 5 days. According to the test procedure the animals were killed 6, 4 and 48 hours (single exposure) or 6 h (5-day exposure) after administration.
Ethyl methanesulfonate (EMS) was used as positive control substance and administered orally.
Diethylene glycol dimethyl ether did not produce a detectable significant aberration of the bone marrow metaphase chromosomes of rats.
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