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Key results on acute aquatic toxicity are as follows:

- Fish: Leuciscus idus (golden orfe), LC50 (96 hours) = 146.6 mg/L, obtained with 3-methoxypropylamine (CAS 5331 -73 -0)

- Aquatic invertebrates: Daphnia magna, EC50 (48 hours) = 65 mg/L, obtained with the related substance 3-isopropoxypropylamine (CAS 2906 -12 -9)

- Algae: Pseudokirchneriella subcapitata, ErC50 (72 hours) = 44 mg/L and ErC10 (72 hours) = 29 mg/L, obtained with the related substance 3-isopropoxypropylamine

- Aquatic microorganisms: Pseudomonas putida, EC10 (16 hours) = 82.5 mg/L, obtained with the related substance 2-ethoxyethylamine (CAS 110 -76 -9)

Based on the results from all available studies with these three related substances, it can be concluded that the pH increasing effect of the substances in the test solutions was in certain cases completely and in certain cases partly responsible for the observed adverse effects. Because not in all tests a clear distinction could be made between the contribution of the pH increase and the contribution of potential direct intrinsic toxic effects (if any), as a worst case, the key effect concentrations mentioned above were those obtained in non-neutralised test solutions.

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