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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1990
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report Date:
1990

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Version / remarks:
EG-Guideline 84/449 C1 comparable to OECD 203
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
- Name of test material: Marlon PS 65
- Chemical name: Sekundäres C13-17 Alkansulfonat Na-Salz ( Secundary alkane sulfonate -Na-salt)
- Analytical purity: 66 +/- 0.5 %
- Lot/batch No.: 3630/81235 )(18.09.1990)

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Leuciscus idus melanotus
Details on test organisms:
TEST ORGANISM
- Common name: Goldenorfe
- Strain: Leuciscus idus melanotus Heckel
- Source: Fischzucht Paul Eggers, Hoheweststadt
- Length at study initiation: 6 +/- 2 cm
- Method of breeding: Housing in dechlorinated tap water, in flow -trough 200L-stone tanks at a temperature of 22 +/- 2 °C.
- Type and amount of food: Tetramin, 3 % of bodyweight/day.
- Feeding frequency: daily

- Feeding during test: No

Study design

Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
15 °d
Test temperature:
21 +/- 1°C
pH:
7.7 - 8.1
Dissolved oxygen:
7.4 - 9.0
Nominal and measured concentrations:
measured concentrations: control, 3.2, 7.1, 9.9, 14.7 and 20.3 mg/L (at test start)
measured concentrations: control, 0.6, 2.6, 7.8, 10.87 and 20.0 mg/L (at test end)
Details on test conditions:
TEST SYSTEM
- Test vessel: 45L-tanks
- Material, size, headspace, fill volume: 35 L
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
- Intervals of water quality measurement: at 0, 24, 48 and 72 hours


OTHER TEST CONDITIONS
- Photoperiod: 16/8 light dark cycle


EFFECT PARAMETERS MEASURED : mortality after 24, 48, 72 and 96 hours

Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
5.5 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
act. ingr.
Basis for effect:
mortality
Remarks on result:
other: 65% a.i. in test mat.
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
8.4 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
7.1 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality

Any other information on results incl. tables

Nominal conc.(mg/l)

Number of fish

Mortality (%) after

24 h

48 h

72 h

96 h

control

10

0

0

0

0

3.2

10

0

0

0

0

7.1

10

0

0

0

0

9.9

10

100

100

100

100

14.7

10

100

100

100

100

20.1

10

100

100

100

100

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
In this study the test substance was found to be toxic to Leuciscus idus melanotus after 96 hrs at a measured test item concentration of 9.9 mg/L and higher. Therefore, the NOEC was laid down as 7.1 mg/L (measured test item concentration). The LC50 (96 hrs) is 8.4 mg/L
Executive summary:

The acute toxicity of the test item Secondary alkane sulfonate, sodium salt to Leuciscus idus melanatus was determined in a 96 -hour flow-through test according to the EU Commission Directive 84/449 C1.

The concentrations of the test item of 3.2, 7.1, 9.9, 14.7 and 20.3 mg/L were tested in parallel with a control. There was no mortality at the control group. The highest tested concentration without an effect was 7.1 mg/L. The calculated LC50 was 8.4 mg/L (arithmetic mean). The lowest tested concentration with 100 % effect was 9.9 mg/L.

Up to a concentartion of 7.1 mg/L no mortality was observed.