Registration Dossier
Registration Dossier
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EC number: 215-242-4 | CAS number: 1314-80-3
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Published data by the US Environmental Protection Agency.
Data source
Reference
- Reference Type:
- publication
- Title:
- Validation of Chemical and Biological Techniques for Evaluation of Vapors in Ambient Air Testing of Twelve Vapor-Phase Compounds
- Author:
- Huges TJ, Sparacino C & Frazier S
- Year:
- 1 984
- Bibliographic source:
- Research Triangle Park NC, Environmental Protection Agency, Health Effects Research Laboratory, Apr 1984
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Bacterial mutagenicity (Ames test in S. typhimurium)
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Hydrogen sulphide
- EC Number:
- 231-977-3
- EC Name:
- Hydrogen sulphide
- Cas Number:
- 7783-06-4
- Details on test material:
- The test material was one of twelve substances tested for mutagenicity.
Constituent 1
Method
- Target gene:
- Various
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 97
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- not specified
- Species / strain / cell type:
- S. typhimurium TA 98
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- not specified
- Species / strain / cell type:
- S. typhimurium TA 100
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor-induced hamster and rat liver S9
- Test concentrations with justification for top dose:
- The highest dose was 1750 µg/plate as the limiting factor was that hydrogen sulphide gas was limited by its solubility in ethanol, which was the test solvent.
- Vehicle / solvent:
- Ethanol
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- not specified
- Details on test system and experimental conditions:
- Salmonella typhimurium TA97, TA98 and TA100 strains, either with or without S9 liver fraction of male rats or hamsters that had been induced with 500 mg/kg Arcoclor.
- Evaluation criteria:
- Not stated
- Statistics:
- Not stated
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 97
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not determined
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not determined
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not determined
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Additional information on results:
- No further information.
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
No evidence of mutagenicity was observed in the Ames test with Salmonella typhimurium TA97, TA98 and TA100.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
No mutagenicity was observed in the Ames test with Salmonella typhimurium TA97, TA98 and TA100. - Executive summary:
No evidence of mutagenicity was observed in the Ames test with Salmonella typhimurium TA97, TA98 and TA100.
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