Trifluoro(trifluoromethoxy)ethylene

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: guideline test with GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Deviations:

yes

Remarks:

adaptation to gaseous form

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Trifluoro(trifluoromethoxy)ethylene

EC Number:

214-703-7

EC Name:

Trifluoro(trifluoromethoxy)ethylene

Cas Number:

1187-93-5

Molecular formula:

C3F6O

IUPAC Name:

1,1,2-trifluoro-2-(trifluoromethoxy)ethene

Test material form:

other: gas

Details on test material:

A cylinder containing 153g of the test material, Ethene, trifluoro(trifluoromethoxy), Cylinder No. 1199D, was received at IRI on 8 April 1987. The test material, a gas (boiling point -22℃), was stored in the dark under ambient conditions.

Test animals / tissue source

Species:

rabbit

Strain:

New Zealand White

Details on test animals or tissues and environmental conditions:

Two male and one female rabbits(weight range 2.5-3.0kg)were used. They were housed individually in aluminium cages with grid floors beneath which were peat moss filled trays. They were fed on Special Diet Services Rabbit Diet of satisfactory analysis and allowed food and water ad libitum. A 12 h light/dark cycle was in operation. Mean environmental maximum and minimum temperatures were 19℃ and 16℃ and mean relative humidity was 54%. The animals were allowed an acclimatisation period of 63 days before test commencement.

Test system

Vehicle:

not specified

Controls:

other: the other eye remained untreated to serve as a control

Amount / concentration applied:

in a single burst

Duration of treatment / exposure:

1 second

Observation period (in vivo):

at 1, 24, 48 and 72 h after instillation

Number of animals or in vitro replicates:

two males and one female

Details on study design:

The rabbit was held firmly but gently and the right eye was held open. The test material was then administered in a single burst of about 1 s duration from a distance of 10 cm directly in front of the eye. The lids were then gently held together for 1 or 2s. The other eye remained untreated to serve as a control. The eyes were examined for irritation, using standard illumination, at 1, 24, 48 and 72h ater instillation, using the scoring system as OECD 405.

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

cornea opacity score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Remarks on result:

no indication of irritation

Results 2

Irritation parameter:

iris score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Remarks on result:

no indication of irritation

Results 3

Irritation parameter:

conjunctivae score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Remarks on result:

no indication of irritation

Results 4

Irritation parameter:

chemosis score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Remarks on result:

no indication of irritation

Other effects:

No discharge observed

Any other information on results incl. tables

See data reported in the field "Attached background material".

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

It can be concluded that the test substance is non-irritant to rabbit eyes.

Executive summary:

Two male and one female rabbits were used to conduct this acute eye irritation test. The test material was administered in a single burst of about 1 second duration .The eyes were examined for irritation. The reaction scores for cornea, iris, and conjunctivae in this test are 0. No corneal, iridial or conjunctival responses were noted. Therefore, it can be concluded that the test substance shall not be classified according to CLP (Regulation EC No.1272/2008).