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Short-term toxicity to fish

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Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
1992-03-23 to 1992-04-05
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP guideline study without analytics according to OECD 203
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
yes
Remarks:
, no analytical dose verification
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: required amounts of the test substance were placed in a 1 L glass cylinder and made up to 1 L with water and added to 9 L of test water. Highest concentration of 1000 mg/L (for the preliminary test) was prepared directly by dissolving test item in the final volume of 10 L dechlorinated tap water
- Controls: Blank control with test medium only
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): none
- Stock solution: prepared directly by dissolving test item in the final volume of 10 L dechlorinated tap water.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): none
Test organisms (species):
Leuciscus idus
Details on test organisms:
TEST ORGANISM
- Common name: Golden orfe
- Source: Fischzucht Eggers / D-2354 Hohenwestedt
- Length at study initiation (length definition, mean, range and SD): 5.9 cm (mean of 20 fish
- Weight at study initiation (mean and range, SD): 2.22 g (mean of 20 fish)
- Method of holding:
- temperature: 18 ± 2.0 °C
- oxygen: at least 80% of air saturation (ca. 8.0 mg O2/L)
- pH: 7.0 - 8.5
- hardness: 14 °dH
- light: 16h daily; intensity: 600-800 lux
- feeding: once daily with trout pellets
- Feeding during test: none


ACCLIMATION
- Acclimation period: minimum of 14 days prior to test initiation
- Acclimation conditions (same as test or not): no (250 L glass container, flow-through conditions and permanent aeration)
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
14°dH
Test temperature:
18. 0 - 19.0°C
pH:
8.11 - 8.32
Dissolved oxygen:
> 60%
(9.6 - 11 mg/L O2)
Nominal and measured concentrations:
Nominal test substance concentrations: 10, 18, 32, 56, and 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Glass aquaria of 12 liters
- Aeration: continuous aeration using a membrane pump system and pasteur pipettes for contact with test medium
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 10 L for 5 fish

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water (from SG dechlorination system)
- Intervals of water quality measurement: Daily

OTHER TEST CONDITIONS
- Photoperiod: 16 hours daily (600 - 800 Lux)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality, Symptoms

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.78 (geometric series)
- Range finding study
- Test concentrations: 0.1, 1, 10, 100 and 1000 mg/L
- Results used to determine the conditions for the definitive study: LC100 (96 h): 100 mg/L, LC0 (96 h): 10 mg/L
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
32 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
72.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
32 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Behavioural abnormalities: None
- Mortality of control: None
- Other adverse effects control: None
- Abnormal responses: None
- Any observations (e.g. precipitation): None
Reported statistics and error estimates:
48 - 96 h LC50 values determined graphically as calculation of LC50 value by means of Probit analysis according to Finney was not feasible.

Due to the distribution of the data, the calculation of the LC50 values by means of the Probit analysis according to Finney was not feasible. Therefore, the 48 - 96 h LC50 was determined graphically.

Table: Mortality in preliminary test

Conc. Mg/L

1000

100

10

1

0.1

control

Mortality

%

%

%

%

%

%

2-4 h

0

0

0

0

0

0

24 h

100

40

0

0

0

0

48 h

100

100

0

0

0

0

72 h

100

100

0

0

0

0

96 h

100

100

0

0

0

0

Table: Mortality in main test

Conc. Mg/L

100

56

32

18

10

control

Mortality

%

%

%

%

%

%

2-4 h

0

0

0

0

0

0

24 h

0

0

0

0

0

0

48 h

100

10

0

0

0

0

72 h

100

10

0

0

0

0

96 h

100

10

0

0

0

0

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
April - May 1996
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: OECD Guideline Study performed in compliance with the UK Principles of Good Laboratory Practice, no analytical monitoring
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test solutions were prepared separately by adding the required amount of test substance to dechlorinated tap water.
Test organisms (species):
Leuciscus idus
Details on test organisms:
TEST ORGANISM
- Common name:Juvenile golden orfe.
- Source:Fish were obtained from Xotic Fish Centre, Alfreton, Derbyshire
- Length at study initiation (length definition, mean, range and SD): 5.4 cm (SD: 0.3)
- Weight at study initiation (mean and range, SD): 1.54 g (SD: 0.38)


ACCLIMATION
- Acclimation period: 1 week
- Acclimation conditions (same as test or not): 21± 1 °C >=8.3 mg O2/L
- Type and amount of food during acclimation:Tetramin flake food.
- Feeding frequency during acclimation:feeding was stopped 48 hours prior to test start.
- Health during acclimation (any mortality observed): no mortality observed
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
100 mg/L as CaCO3
Test temperature:
20.0 - 20.5
pH:
6.2 - 7.9 (at concentrations 100 - 320 mg/L)
3.6 (at concentration 560 mg/L)
2.6 (at concentration 1000 mg/L)
Dissolved oxygen:
7.9 - 8.0 mgO2/L
Nominal and measured concentrations:
Nominal: 100 mg/L, 180 mg/L, 320 mg/L, 560 mg/L and 1000 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: glass aquaria (20 L)
- Type (delete if not applicable): covered to reduce evaporation
- Material, size, headspace, fill volume:
- Aeration: yes
- Renewal rate of test solution (frequency/flow rate): daily
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.77 g bodyweight/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water dechlorinated by passage through an activated carbon filter.
- Behavioural abnormalities:
OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16:8 h day-night regime

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and adverse reactions at 3, 6, 24, 48, 72 and 96 hours

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 1, 10, 100 and 1000 mg/L
- Results used to determine the conditions for the definitive study: Mortalities observed at 1000 mg/L
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
420 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 320 - 560 mg/L
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
180 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Behavioural abnormalities: swimming at the surface at concentrations > 180 mg/L.

Cumulative mortality

 Nominal substance concentrations [mg/L]  3 h 6 h   24 h  48 h 72 h   96 h
 0  0  0  0
 100  0  0  0
 180  0  0  0
320  0  0  0
 560  6 10 10  10  10  100
 1000  10  10  10  10  10  100

Description of key information

Key value for chemical safety assessment

LC50 for freshwater fish:
72.5 mg/L

Additional information

The acute toxicity on fish was tested for the parent substance 1,3 -propanesultone and for the product of hydrolysis 3-hydroxypropanesulphonic acid. Due to the rapid hydrolysis testing of the parent substance 1,3-propanesultonewill ultimately result in an exposure to the product of hydrolysis. Therefore the available tests were assessed in a weight of evidence approach.

The acute toxicity to Leuciscus idus was tested in a guideline study following OECD 203, conducted with the test item 3-hydroxypropanesulphonic acid. The fish were exposed to nominal substance concentrations of 100 mg/L, 180 mg/L, 320 mg/L, 560 mg/L and 1000 mg/L. The test was set up in a semi-static test design with a daily renewal rate of the tested concentrations. After a total exposure period of 96 hours a LC50 (96 h) of 420 mg/L based on nominal concentrations was determined.

The acute toxicity of 1,3-propanesultone was tested in a static test according to OECD 203. Fish of the species Leuciscus idus were exposed to nominal test item concentrations of 0 (control), 10, 18, 32, 56 and 100 mg/L.  The 96-h LC50 was 72.5 mg/L.