N,N'-bis(3-aminopropyl)ethylenediamine

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

disregarded due to major methodological deficiencies

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable study report wich meets basic scientific principles. Not relevant for REACH.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1977

Report date:

1977

Materials and methods

Principles of method if other than guideline:

BASF test:
Aqueous solutions of the test substance were injected into the peritoneal cavity of the mice. Group-wise documentation of clinical signs was performed over the 14-day study period. Body weight was determined before the start of the study only, as it was needed for determination of dose. The clinical signs and findings were reported in summary form.

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

N,N'-bis(3-aminopropyl)ethylenediamine

EC Number:

234-147-9

EC Name:

N,N'-bis(3-aminopropyl)ethylenediamine

Cas Number:

10563-26-5

Molecular formula:

C8H22N4

IUPAC Name:

(3-aminopropyl)({2-[(3-aminopropyl)amino]ethyl})amine

Details on test material:

- Name of test material (as cited in study report): N-4 Amin
- Analytical purity: > 97 %

Test animals

Species:

mouse

Strain:

NMRI

Sex:

male/female

Details on test animals or test system and environmental conditions:

- Weight at study initiation: male 18.4 g, females 20.4 g (mean)
- Diet: Altromin R 124 (Altromin GmbH, Lage)

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

water

Doses:

100, 147, 178, 215 and 316 ml/kg bw (corresponding to 95, 140, 169, 204 and 300 mg/kg bw)

No. of animals per sex per dose:

5

Control animals:

no

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 171 mg/kg bw

Mortality:

Mortality occured within 24 hours in the 204 and 300 mg/kg bw dose groups after injection of the test substance.

Clinical signs:

Dyspnoea, tremor, tonic convulsions, apathy, abdominal position, poor general state, spastic gait.

Body weight:

Body weight gain was generally observed in the course of the study.

Gross pathology:

No abnormalities were noted at necropsy of animals died during the study or sacrificed at the end of the study.

Any other information on results incl. tables

Dose (mg/kg bw)	Mortality Dead/Treated
	1 hour		24 hours		48 hours		7 days		14 days
	male	female	male	female	male	female	male	female	male	female
95	0/5	0/5	0/5	0/5	0/5	0/5	0/5	0/5	0/5	0/5
140	0/5	0/5	0/5	0/5	0/5	0/5	0/5	0/5	0/5	0/5
169	0/5	0/5	0/5	0/5	0/5	0/5	0/5	0/5	0/5	0/5
204	0/5	0/5	0/5	3/5	3/5	3/5	3/5	4/5	3/5	4/5
300	0/5	0/5	5/5	5/5	5/5	5/5	5/5	5/5	5/5	5/5