N,N'-bis(3-aminopropyl)ethylenediamine

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

26 June 1989 to 28 June 1989

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Strain: Institut National Recherche Chimique Appliqué, France
- Source: originally supplied from 'Institut National Recherche Chimique Appliqué, France' and has been bred from 1978 in the laboratories of BASF
- Age at study initiation (mean and range, SD): 2 to 24 h
- Feeding during test: no

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

2.7 ± 0.5 mM

Test temperature:

18.85 - 20.85 °C

pH:

0 h: 8.14 to 9.85
48 h: 8.01 to 8.39

Dissolved oxygen:

8.01 to 8.67 mg/L

Nominal and measured concentrations:

Nominal: Control, 6.25, 12.5, 25, 50, 100, 200 mg/L; 200 mg/L (neutralised)

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: centrifuge glass tubes with flat bottom; fill volume: 10 mL
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 2 mL/animal

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconsituted freshwater (mixture of salts added to deionised water)
- Ca/mg ratio: 4:1
- Conductivity: 600 - 700 µS/cm
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light : 8 hours darkness
- Light intensity: diffuse

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- mobility: 0, 3, 6, 24, 48 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Justification for using less concentrations than requested by guideline: not applicable

Duration:

48 d

Dose descriptor:

EC50

Effect conc.:

42.54 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 30.18 to 54.9 mg/L; based on not pH-adjusted test solutions

Duration:

48 d

Dose descriptor:

EC0

Effect conc.:

25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: based on not pH-adjusted test solutions

Duration:

48 d

Dose descriptor:

EC100

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: based on not pH-adjusted test solutions

Details on results:

- Mortality of control: 0%
- The highest tested concentration (200 mg/L) was also tested in parallel after pH adjustment. No significant changes were noted compared to the not pH-adjusted test solution.

Cummulative immobility

Nominal conc. (mg/L)	Immobilised animals (n; cumulated)
	0 h	3 h	6 h	24 h	48 h
0	0	0	0	0	0
6.25	0	0	0	0	0
12.5	0	0	0	0	0
25	0	0	0	0	0
50	0	0	0	15	20
100	0	0	0	15	20
200	0	0	0	20	20

pH values

Nominal conc. (mg/L)	pH after
	0 h	48 h
0	8.14	8.01
6.25	8.39	8.13
12.5	8.64	8.14
25	8.93	8.21
50	9.24	8.32
100	9.59	8.33
200	9.85	8.39

Dissolved oxygen

Nominal conc. (mg/L)	Dissolved oxygen (mg/L)
	0 h	48 h
0	8.11	8.61
6.25	8.06	8.66
12.5	8.02	8.67
25	8.04	8.63
50	8.12	8.60
100	8.02	8.52
200	8.01	8.56

Description of key information

Acutely harmful to aquatic invertebrates

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

42.54 mg/L

Additional information

The acute toxicity of N,N'-bis(3 -aminopropyl)ethylenediamine (CAS 10563 -26 -5) was studied in a static test according to method C2 of Annex V of the EU Directive 79/831/EEC. The test item concentrations were not analytically verified, but are assumed to be stable regarding the sufficient recovery (> 80%) in the chronic study with Daphnia magna (BASF SE, 2014). The 48-h EC50 was determined to be 42.54 mg/L (result based on not pH-adjusted test solutions). In addition to the not pH-adjusted test solutions, the highest test concentration of 200 mg/L was tested in parallel after neutralisation. The toxic effect was similar to the not-adjusted test solution (BASF AG, 1991; report no. 1/0502/2/89 -0502/89).