3,4-dihydro-2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-2H-benzopyran-6-yl acetate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in mammalian cells

Remarks:

Type of genotoxicity: gene mutation

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well-documented publication which meets basic scientific principles.

Data source

Reference

Reference Type:

publication

Title:

Mutagenicity of Tocopheryl Quinones: Evolutionary Advantage of Selective Accumulation of Dietary alpha-Tocopherol.

Author:

Cornwell DG et al

Year:

2002

Bibliographic source:

Nutr Cancer 43 (1): 111-118

Materials and methods

Principles of method if other than guideline:

Method: other: according to Tindall KR and Stankowski LF (1989). Mutat Res 220: 241-253

GLP compliance:

not specified

Type of assay:

mammalian cell gene mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

3,4-dihydro-2,7,8-trimethyl-2-(4,8,12-trimethyltridecyl)-2H-benzopyran-6-ol

EC Number:

231-523-4

EC Name:

3,4-dihydro-2,7,8-trimethyl-2-(4,8,12-trimethyltridecyl)-2H-benzopyran-6-ol

Cas Number:

7616-22-0

IUPAC Name:

2,7,8-trimethyl-2-(4,8,12-trimethyltridecyl)chroman-6-ol

Details on test material:

d-gamma-tocopherol; according to the authors, purity was 92.6% (no data on contaminants)

Method

Species / strain

Species / strain / cell type:

other: Chinese hamster ovary AS52 cells

Additional strain / cell type characteristics:

other: AS52 cells: cells which lack the normal X-like mammalian hypoxanthine-guanine phosphoryltransferase (hprt) gene but contain a single functional copy of the E coli xanthin- guanine phosphoribosyltransferase (gpt) gene stably integrated into the genome.

Metabolic activation:

without

Test concentrations with justification for top dose:

ca. 2.9, 14.6 ug/ml (6.8, 34 uM)

Details on test system and experimental conditions:

Cells were incubated with the test substance at concentrations of 6.8 and 34 uM (ca. 2.9 and 14.6 ug/ml) for 5 hours.
Mutagenicity was expressed as an increase in the number of thioguanine-resistant (TGr) clones; cytotoxicity was determined by measuring the cloning efficiency. Untreated controls cultured in medium and solvent controls treated with the vehicle (ethanol) were included.

Results and discussion

Test results

Metabolic activation:

without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

other: ca. 14.6 ug/ml (34 uM)

Additional information on results:

The test substance had no effect on mutant frequency. A significant decrease in cloning efficiency was observed at the high concentration when compared with controls; relative cloning efficiency was approximately 85-90% (control = 100%). The results of the cytotoxicity study are presented only graphically; no further data.

Remarks on result:

other: other: Chinese hamster ovary AS52 cells

Remarks:

Migrated from field 'Test system'.