Tricobalt dicitrate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro cytogenicity / micronucleus study

Remarks:

Type of genotoxicity: chromosome aberration

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Basic data given.

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

publication

Title:

Micronucleus test and erythropoiesis: Effect of cobalt on the induction of micronuclei by mutagens.

Author:

Suzuki Y. et al.

Year:

1993

Bibliographic source:

Environmental and molecular mutagenesis 22: 101-106

Materials and methods

Principles of method if other than guideline:

CoCl2*6H2O was tested for its ability to induce micronuclei using mouse bone marrow cells cultured in vitro.

GLP compliance:

not specified

Type of assay:

in vitro mammalian cell micronucleus test

Test material

Test material information

Constituent 1

Reference substance name:

7791-13-1

EC Number:

616-574-6

Cas Number:

7791-13-1

IUPAC Name:

7791-13-1

Constituent 2

Reference substance name:

cobalt(II) dichloride hexahydrate

IUPAC Name:

cobalt(II) dichloride hexahydrate

Details on test material:

- Name of test material (as cited in study report): cobalt(II)chloride hexahydrate
- Molecular formula (if other than submission substance): CoCl2*6H2O
- Molecular weight (if other than submission substance): 238 g/mol
- Analytical purity: no data

Method

Species / strain

Species / strain / cell type:

primary culture, other: mouse bone marrow cells

Additional strain / cell type characteristics:

not specified

Metabolic activation:

with and without

Metabolic activation system:

S9 mix: S9 was prepared from BALB/c mouse liver induced by polychlorinated biphenyls [KC500]

Test concentrations with justification for top dose:

0, 1, 5, 10, 50 µg/mL

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: water

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

no

Positive control substance:

no

Statistics:

The data for micronucleus induction were analysed statistically by the Kastenbaum-Bowman method [Kastenbaum and Bowman, 1970]. Data for the P/N ratio were analysed statistically using student´s t-test and one-way layout analysis of variance.

Results and discussion

Test results

Species / strain:

primary culture, other: mouse bone marrow cells

Metabolic activation:

with and without

Genotoxicity:

negative

Remarks:

No positive control substance was used to validate the test assay.

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

valid

Untreated negative controls validity:

not examined

Positive controls validity:

not examined

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

The frequency of MPCE and P/N ratio in the in vitro micronucleus test was not significantly altered by treatment with the test item in the presence or absence of S9 mix. No positive control substance was used to validate the test assay.

Without S9 mix

Concentration of CoCl2*6H2O [µg/mL]	MPCE frequency [%]	P/N ratio
50	0.40±0.40	1.24±0.33
10	0.30±0.20	1.38±0.22
5	0.40±0.20	1.31±0.48
1	0.20±0.00	1.40±0.07
0	0.33±0.30	1.34±0.16

Results show mean ± SD

 

With S9 mix

Concentration of CoCl2*6H2O [µg/mL]	MPCE frequency [%]	P/N ratio
50	0.67±0.31	0.91±0.09
10	0.66±0.53	0.90±0.13
5	0.53±0.43	0.80±0.06
1	0.27±0.37	1.00±0.04
0	0.53±0.12	0.85±0.07

Results show mean ± SD

 

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative