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EC number: 203-815-1 | CAS number: 110-91-8
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- Uses advised against
- Endpoint summary
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- Endpoint summary
- Stability
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
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- Endocrine disrupter testing in aquatic vertebrates – in vivo
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- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- no data available
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-compliant study performed according to OECD Guideline 405 (Acute Eye Irritation / Corrosion).
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 985
- Report Date:
- 1985
Materials and methods
Test guideline
- Qualifier:
- according to
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Deviations:
- not applicable
- GLP compliance:
- yes
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Details on test material:
- - Name of test material: Morpholine
- Analytical purity: 99 %
Test animals / tissue source
- Species:
- rabbit
- Strain:
- not specified
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Housing: individual in cages
- Diet: controlled access
- Water: ad libitum
- Acclimation period: appr. one week
ENVIRONMENTAL CONDITIONS
- Temperature: 20 ± 3 °C
- Humidity: 50 ± 20 %
- Photoperiod: 12 hours dark/12 hours light
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: the adjacent eye served as control.
- Amount / concentration applied:
- TEST MATERIAL
- Amount applied: 0.1 mL
- Concentration: undiluted - Duration of treatment / exposure:
- single application,
exposure period: 4 seconds, 30 seconds, 24 hours - Observation period (in vivo):
- 21 days
- Number of animals or in vitro replicates:
- 3 animals per exposure period
- Details on study design:
- REMOVAL OF TEST SUBSTANCE
If necessary, the treated eye was washed with tap water, at least 24 hous after treatment.
SCORING SYSTEM: according to OECD GL 405
TOOL USED TO ASSESS SCORE: biomicroscope / fluorescein / ophthalmoscope
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- conjunctivae score
- Remarks:
- (exposure 4 s)
- Basis:
- mean
- Time point:
- other: 24 h, 48 h, 72 h
- Score:
- >= 2 - <= 2.3
- Max. score:
- 3
- Irritation parameter:
- conjunctivae score
- Remarks:
- (exposure 30 s)
- Basis:
- mean
- Time point:
- other: 24 h, 48 h, 72 h
- Score:
- >= 2 - <= 2.7
- Max. score:
- 3
- Irritation parameter:
- conjunctivae score
- Remarks:
- (exposure 24 h )
- Basis:
- mean
- Time point:
- other: 24 h, 48 h, 72 h
- Score:
- >= 2.7 - <= 3
- Max. score:
- 3
- Irritation parameter:
- chemosis score
- Remarks:
- (exposure 4 s)
- Basis:
- mean
- Time point:
- other: 24 h, 48 h, 72 h
- Score:
- >= 1 - <= 2.3
- Max. score:
- 4
- Irritation parameter:
- chemosis score
- Remarks:
- (exposure 30 s)
- Basis:
- mean
- Time point:
- other: 24 h, 48 h, 72 h
- Score:
- >= 1 - <= 2.3
- Max. score:
- 4
- Irritation parameter:
- chemosis score
- Remarks:
- (exposure 24 h)
- Basis:
- mean
- Time point:
- other: 24 h, 48 h, 72 h
- Score:
- >= 2.7 - <= 3.7
- Max. score:
- 4
- Irritation parameter:
- cornea opacity score
- Remarks:
- (exposure 4 s)
- Basis:
- mean
- Time point:
- other: 24 h, 48 h, 72 h
- Score:
- >= 1.7 - <= 2.3
- Max. score:
- 4
- Irritation parameter:
- cornea opacity score
- Remarks:
- (exposure 30 s)
- Basis:
- mean
- Time point:
- other: 24 h, 48 h, 72 h
- Score:
- >= 1.3 - <= 2
- Max. score:
- 4
- Irritation parameter:
- cornea opacity score
- Remarks:
- (exposure 24 h)
- Basis:
- mean
- Time point:
- other: 24 h, 48 h, 72 h
- Score:
- >= 1.3 - <= 1.7
- Max. score:
- 4
- Irritation parameter:
- iris score
- Remarks:
- (exposure 4 s)
- Basis:
- mean
- Time point:
- other: 24 h, 48 h, 72 h
- Score:
- >= 0.5 - <= 0.7
- Max. score:
- 2
- Irritation parameter:
- iris score
- Remarks:
- (exposure 30 s)
- Basis:
- mean
- Time point:
- other: 24 h, 48 h, 72 h
- Score:
- >= 0.3 - <= 0.5
- Max. score:
- 2
- Irritation parameter:
- iris score
- Remarks:
- (exposure 24 h)
- Basis:
- mean
- Time point:
- other: 24 h, 48 h, 72 h
- Score:
- >= 0 - <= 0.3
- Max. score:
- 2
Any other information on results incl. tables
All expositions with Morpholine (for 4 s, 30 s, and 24 h) resulted in eye irritation of animals. In addition, rabbits showed necrosis around the eyes. After an exposure time of 24 hours burns (corrosion) were observed. In 2 rabbits corrosion was noted both inside and around the eyes after 30 seconds of exposure. After an observation period of 21 days eye injuries were still observed in one animal.
Exposure group 24 hours:
All animals showed necrosis in the eye and loss of fur, sores, redness, and scarring on the front of the upper eyelids outside. In one animal eye injuries were cleared within 10 days and detachment of the outer layers of the cornea were observed.
Exposure group 30 s:
In all animals whitish secretions were observed both in and around the eyes.In 2/3 animals eye injuries were cleared within 10 days and detachment of the outer layers of the cornea were observed. In the 2 animals where corrosion was noted, hair loss was also observed.
Exposure group 4 s:
In all animals whitish secretions were observed both in and around the eyes. In 2/3 animals eye injuries were cleared within 10 days and detachment of the outer layers of the cornea were observed. One rabbit showed a pale flash film, perhaps with scars.Applicant's summary and conclusion
- Interpretation of results:
- corrosive
- Remarks:
- Migrated information
- Executive summary:
- In a primary eye irritation study (Koch et al., 1985), 100 µL of undiluted Morpholine (99 %) was instilled into the conjunctival sac of the left eye of 3 rabbits. The eyes were washed out after 24 hours. Further exposure times were 4 and 30 seconds, respectively (3 rabbits/exposure time). Animals were observed for 21 days. Irritation was scored according to OECD guideline 405. All three expositions gave rise to eye irritation. All animals showed necrosis in and around the eyes. After an exposure time of 24 hours burns (corrosion) were observed. Two of the animals also gave rise to corrosion both inside and around the eyes at 30 seconds of exposure. After the observation period eye injuries were still observed in one animal. The authors classified Morpholine as irritant substance; however, corrosive effects in several animals were observed. Thus, Morpholine can be regarded as a corrosive substance. This study is classified acceptable. It satisfies the requirement of OECD test guideline 405.
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