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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
03. Jan 1967 - 11. Jan 1967
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1967
Report date:
1967

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
Deviations:
not specified
Principles of method if other than guideline:
BASF Test, see details in remarks on material and methods
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Morpholine
EC Number:
203-815-1
EC Name:
Morpholine
Cas Number:
110-91-8
Molecular formula:
C4H9NO
IUPAC Name:
morpholine
Test material form:
liquid

Test animals

Species:
rabbit
Strain:
Vienna White
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 3.22 and 2.94 kg

Test system

Type of coverage:
occlusive
Preparation of test site:
clipped
Vehicle:
unchanged (no vehicle)
Controls:
other: untreated skin of the same animals served as control.
Duration of treatment / exposure:
1, 5, 15 min or 20 hours
Observation period:
8 days
Number of animals:
2 animals
Details on study design:
TEST SITE
- Area of exposure: 2.5 x 2.5 cm

REMOVAL OF TEST SUBSTANCE
- Washing (if done): conc. Lutrol and 50 % Lutrol solution
- Time after start of exposure: 1, 5 or 15 min post exposure

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
overall irritation score
Remarks:
1 min exposure
Basis:
mean
Time point:
other: 8 days
Score:
1
Max. score:
3
Reversibility:
not reversible
Remarks on result:
positive indication of irritation
Irritation parameter:
overall irritation score
Remarks:
5 and 15 minutes
Basis:
mean
Time point:
other: 8 days
Score:
2
Max. score:
3
Reversibility:
not reversible
Remarks on result:
positive indication of irritation
Irritation parameter:
erythema score
Basis:
mean
Time point:
24/48/72 h
Remarks on result:
not measured/tested
Irritation parameter:
edema score
Basis:
mean
Time point:
24/48/72 h
Remarks on result:
not measured/tested
Irritant / corrosive response data:
The application of the test substance for 1 min caused in 1 animal haemorrhagic areas and leathery-like necrosis at the end of the observation period.
The application for 5 min caused after 24 hours parchment-like necrosis in 1 animal and haemorrhagic areas after 24 hours and leathery-like necrosis after 8 days.
The application of the test substance for 15 min caused haemorrhagic areas, oedema and parchment-like necrosis after 24 hours which declined to leathery-like necrosis after 8 days.
Leathery-like necrosis was considered as a full-thickness necrosis.

Applicant's summary and conclusion

Interpretation of results:
Category 1 (corrosive) based on GHS criteria