Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 213-195-4 | CAS number: 929-06-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EPA OPP 84-2
- GLP compliance:
- yes
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- 2-(2-aminoethoxy)ethanol
- EC Number:
- 213-195-4
- EC Name:
- 2-(2-aminoethoxy)ethanol
- Cas Number:
- 929-06-6
- Molecular formula:
- C4H11NO2
- IUPAC Name:
- 2-(2-aminoethoxy)ethanol
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- CD-1
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River laboratories, Kingston,NY
- Diet (e.g. ad libitum): ad libitum (PMI Feeds,Inc. Certified Rodent Diet #5002)
- Water (e.g. ad libitum): ad libitum
- Acclimation period: at least 6 days
ENVIRONMENTAL CONDITIONS
- Temperature : 64-79 °F ( 18-26 °C)
- Humidity (%): 30 - 70
- Air changes (per hr): at least 10 per hour
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- intraperitoneal
- Duration of treatment / exposure:
- The animals were treated once and samples of bone marrow were taken 24 h and 48 h after the treatment.
- Frequency of treatment:
- The animals were treated once.
Doses / concentrations
- Remarks:
- Doses / Concentrations:
62.5, 125, 250 mg/kg
Basis:
- No. of animals per sex per dose:
- 6 male animals per dose
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- cyclophosphamide
Examinations
- Tissues and cell types examined:
- At least 2000 PCEs per animal were analyzed for the frequency of mironuclei. Cytotoxicity was assessed by scoring the numer of PCEs and normochromic erythrocytes (NCEs) in at least 500 erythrocytes for each animals.
- Details of tissue and slide preparation:
- CRITERIA FOR DOSE SELECTION: Pretest were performed
DETAILS OF SLIDE PREPARATION:
Preparation of Slides. Following centrifugation to pellet the tissue, the supematant was removed by aspiration and portions of the pellet were spread on slides and air dried. The slides were fixed in methanol, stained in May-Grünwald Solution followed by Giemsa, and protected by permanently mounted coverslips. For control of bias, all slides were coded prior to analysis.
Slides prepared from the bone marrow collected from five animals per group, if available, at the designated harvest timepoints were scored for micronuclei and the PCE to NCE cell ratio. The micronucleus frequency (expressed as percent micronucleated cells) was determined by analyzing the number of micronucleated PCEs from at least 2000 PCEs per animal. The PCE:NCE ratio was determined by scoring the number of PCEs and NCEs observed scoring at least the first 500 erythrocytes per animal. The historical background frequency of micronucleated cells were expressed as percent micronucleated cells based on the number of PCEs analyzed. The historical background frequency of micronuclei in the Cr1:CD-lB(ICR) BR strain at this laboratory is about 0.0 to 0.4%, which is within the range reported in the published data (Salamone and Mavoumin, 1994). The critena for the identification of micronuclei were those of Schmid (1976). Micronuclei were darkly stained and generally round, although almond- and ring-shaped micronuclei occasionally occurred. Micronuclei were sharp bordered and generally between one-twentieth and one-fifth the size of the PCEs. The unit of scoring was the micronucleated cell, not the micronucleus; thus, the occasional cell with more than one micronucleus was counted as one micronucleated PCE, not two (or more) micronuclei. The staining procedure pennitted the differentiation by color of PCEs and NCEs (bluish-gray and red, respectively).
- Evaluation criteria:
- Assay data analysis was pedormed using an analysis of variance (Winer, 1971) on untransformed proportions of cells with micronuclei per animal and on untransformed PCE:NCE ratios when the variances were homogeneous. Ranked proportions were used for heterogeneous variances. If the analysis of variance was statistically significant (p <0.05), a Dunnett's t-test (Dunnett, 1955; 1964) was used to determine which dose groups, if any, were statistically significantly different from the vehicle control. Analyses were performed separately for each sampling time. The cnteria for a positive response was the detection of a statistically significant increase in micronucleated PCEs for at least one dose level, and a statistically significant dose-related response. A test article that did not induce both of these responses was considered negative. Statistical significance was not the only determinant of a positive response; the Study Director also considered the biological relevante of the results in the final evaluation.
Results and discussion
Test results
- Sex:
- male
- Genotoxicity:
- negative
- Toxicity:
- yes
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
Micronucleus Data Summary Table
Treatment |
Dose |
Harvest Time |
% Micronucleated PCEs mean of 2000a Per animals ± S.E. males |
Ratio PCE:NCE Mean ± S.E. Males |
Controls |
|
|
|
|
Vehicle |
0.5 % CMC |
24 hr |
0.09 ± 0.04 |
0.67 ± 0.09 |
|
|
48 hr |
0.12 ± 0.03 |
0.51 ± 0.02 |
Positive |
CP 80 mg/kg |
24 hr |
1.17 ± 0.12* |
0.55 ± 0.06 |
Test article |
62.5 mg/kg |
24 hr |
0.06 ± 0.02 |
0.61 ± 0.05 |
|
125 mg/kg |
24 hr |
0.06 ± 0.02 |
0.48 ± 0.05 |
|
250 mg/kg |
24 hr |
0.07 ± 0.03 |
0.41 ± 0.04** |
|
|
48 hr |
0.21 ± 0.08 |
0.41 ± 0.05 |
*Significantly greater than the corresponding vehicle control, ps0.01.
** Significantly less than the corresponding vehicle control, ps0.05.
aOne animal from the 24-hour vehicle control group and two animais from the 62.5 mg/kg dose goup were scored out of >2000 PCE/animal. See individual animal data, Table 2.
CMC = Carboxymethy1 cellulose
CP = Cyclophosphamide
PCE = Polychromatic erythrocyte
NCE = Normochromatic erythrocyt
Applicant's summary and conclusion
- Conclusions:
- The test article, Diglycolamine (DGA), was evaluated as negative in the mouse bone marrow micronucleus assay under the conditions of this assay.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

EU Privacy Disclaimer
This website uses cookies to ensure you get the best experience on our websites.