Registration Dossier

Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Abstract

Data source

Reference
Reference Type:
publication
Title:
The Effect of Diacetone Alcohol on the Liver of the Rat
Author:
Keith HM
Year:
1931
Bibliographic source:
Proceedings of the Staff Meetings of the Mayo Clinic, 6, 716-717

Materials and methods

Test animals

Species:
rat

Administration / exposure

Route of administration:
intravenous

Results and discussion

Applicant's summary and conclusion

Executive summary:

The hepatotoxic effects of a single large dose ofdiacetone-alcoholwere studied in rats. Rats were given a single, moderately large intravenous injection ofdiacetone-alcohol,and were observed for 5 weeks. Blood and tissue samples were examined at regular intervals for evidence of liver damage. Hemoglobin and erythrocytes were somewhat reduced initially, but returned to normal values by day 6 post injection. Injury to hepatic cells began within 6 hours after treatment, and reached a maximum at about 24 hours, involving all cells in the lobule. Recovery began at 48 hours and was complete at 7 days. Destruction of Kuppfer cells paralleled that of parenchymal cells. Their recovery, however, seemed to proceed from an extraneous source, and their rapid increase continued for 3 or 4 weeks. In many instances, these proliferating histocytes were arranged into nests of cells scattered along the sinusoids, and some nests contained nucleated erythrocytes after day 21. After 5 weeks, livers were entirely normal. The author concludes that the newly formed histocytes in the liver temporarily assumed hemopoietic functions due to an excessive load on the normal hemopoietic centers.