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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
14 January 1997 - 31 March 1997
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
yes
Details on sampling:
- Frequency: At the start of the exposure and 24 hours after
- Sampling volume: 0.75 mL/ test vessel
Vehicle:
no
Details on test solutions:
Preparation of the test solution (100 mg/L): Five mL of the stock solution was diluted to 5.0 L with dilution water. The stock solution (100000 mg/L) was prepared by adjusting 5000 mg of test substance to 50 mL with pure water.
Dilution water was used for the control group.

Test organisms (species):
Oryzias latipes
Details on test organisms:
TEST ORGANISM
- Common name: Orange killifish
- Source: Sankyo Suisan Co., LTD (1-1 Ichigaya Tamachi, Shinjuku-ku, Tokyo)
- Obtain date: 1 November 1996
- Length at study initiation: 1.80 cm (1.64 - 1.90 cm), n=10
- Weight at study initiation: 0.094 g (0.070 - 0.120 g), n=10
- Feeding during test: No

ACCLIMATION
- Acclimation period: 1 November 1996 - 27 January 1997
- Acclimation conditions: Same as test (Water quality, Temperature, etc.)
- Type and amount of food: Commercial fish food "TetraMin", Not fed for 24 hours prior to the test
- Health during acclimation: Mortality for 7 days prior to the test was 5 % or lower
- Visually healthy and normal fish were used for the test.

OTHER
- Confirming the reproducibility of the test conditions:
- Reference substance: Copper sulfate (II) pentahydrate (Reagent grade, Wako Pure Chemical Industries, Ltd.)
- Results: 96-hr LC50 = 0.44 mg/L and 0.80 mg/L



Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
No data
Test temperature:
24 ± 1 °C
pH:
7.4 - 7.7
Dissolved oxygen:
6.8 - 8.3 mg/L (= 60 % of the O2 saturation conc. (8.25 mg/L at 24.0 °C))
Nominal and measured concentrations:
- Nominal concentrations: 100 mg/L
- Measured concentrations: 98.6 mg/L at the start of the exposure and at 24 hours after
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type: Closed (coverd with teflon sheet to avoid volatilization of the test substance)
- Material: Glass
- Size: 5.0 L
- Fill volume: 5.0 L
- Aeration: No
- Thermostatic bath: PVC Water bath (Thermostat: COOLNIT CL-80F, TAITEC Co., Ltd.)
- Thermomater: 2455 02, Yokogawa Electric Corporation
- D.O. meter: DOL-10, DKK Corporation
- pH meter: HM-40V, TOA Electronics Ltd.
- Renewal rate of test solution: Every 24 hours, the whole quantity
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated water (Yokohama-city tap water, removed the residual chlorine with the activated carbon and aerated sufficiently)
- Hardness: 61 mg/L (as CaCO3)
- pH: 7.8

OTHER TEST CONDITIONS
- Photo period: 16 hours light / 8 hours dark

OBSERVATIONS AND MEASUREMENTS:
- Fish: Mortality and visible abnormality were observed at 24, 48, 72 and 96 hours.
Symptoms should be recorded:
- Death: Touching produces no reaction
- Abnormal breathing: Gill covers move abnormally
- Abnormal swimming: Sluggish or hypersensitive, convulsion, reversed, etc.
- Unable to swim: Just moving at surface or bottom, includs turned over and asphyxia
- Test solutions: Temperature, pH and Dissolved oxygen concentration were measured at the start of the exposure and every 24 hours (before and after renewal of water)

RANGE FINDING STUDY
- Test concentrations: Control, 1.0, 10, 100 mg/L
- No. of replicates: 1
- No. of organisms per group: 5
- Result: The 96h LC50 of the test substance was judged to 100 mg/L or higher, because mortality of 96 hours exposure in 100 mg/L group was 0 %


Reference substance (positive control):
no
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- The median lethal concentration value (LC50) could not be calculated due to the limit test.
- Mortality in 100 mg/L group: 0 %
- Mortality in control group: 0 %

- The highest concentration of 0% mortality at 96 hours: 100 mg/L
- The lowest concentration of 100% mortality at 96 hours: > 100 mg/L

- No toxicological symptom was observed.

- There were no environmental factors which might have affected the reliability of the test.
Reported statistics and error estimates:
The toxicological values were calculated by nominal concentration because the measured concentrations of the test substance during the exposure were kept within ± 20 % of the nominal concentration.

Table 5. Mortality of the Orange killifish (Oryzias latipes) Exposed to the Test Substance

Nominal Concentration
mg/L
MeanaMeasured Concentration
mg/L
Cumulative Mortality (Percent Mortality)
24 Hours 48 Hours 72 Hours 96 Hours
Control ---- 0 (0) 0 (0) 0 (0) 0 (0)
100 98.6 0 (0) 0 (0) 0 (0) 0 (0)

a: geometric mean

Table 6. Calculated LC50 Values

Exposure Period (Hours) LC50 (mg/L) 95-Percent Confidence Limits (mg/L) Statistical Method
24 > 100 -- ~ -- --
48 > 100 -- ~ -- --
72 > 100 -- ~ -- --
96 > 100 -- ~ -- --

Table 7. Observation of the Highest Concentration in 0 % Mortality and the Lowest Concentration in 100 % Mortality Values

Exposure Period (Hours) Highest Concentration in 0 % Mortality (mg/L) Lowest Concentration in 100 % Mortality (mg/L)
24 100 > 100
48 100 > 100
72 100 > 100
96 100 > 100

Table 8. Symptoms of Toxicity Observed in the Orange killifish (Oryzias latipes)

Nominal Concentration
mg/L
MeanaMeasured Concentration
mg/L
Symptoms
24 Hours 48 Hours 72 Hours 96 Hours
Control ---- N N N N
100 98.6 N N N N

a: geometric mean

N: No toxicological symptom was observed

Validity criteria fulfilled:
yes
Remarks:
: Mortality in control group was 0 %. Dissolved oxgen concentration was greater than 60 % of the O2 saturation conc.
Conclusions:
The 96-hr LC50 was greater than 100 mg/L for Oryzias latipes. No mortality occurred up to 100 mg/L.
Executive summary:

Acute toxicity of diacetone alcohol was studied in one acute fish toxicity test. A study with Oryzias latipes was performed in a semi-static system according to OECD TG 203 in compliance with GLP. A limit test was performed at 100 mg/L. Fish were exposed for 96 hour at either the test substance or water control. pH varied between 7.4 and 7.7, dissolved oxygen concentration was between 6.8 and 8.3 mg/L and the temperature was 24 ±1 °C. After 96 hr exposure at the 100 mg/L group, no death was observed. Therefore the 96 -hr LC50 was greater than 100 mg/L.

Description of key information

One acute toxicity study was performed to assess the acute toxicity of DAA to the freshwater fish species Oryzias latipes (Mitsubishi Chemical Safety Institute Ltd., 1997). The study was conducted under GLP and according to OECD Test Guideline 203. A limit test was performed at 100 mg/L. pH varied between 7.4 and 7.7, dissolved oxygen concentration was between 6.8 and 8.3 mg/L and the temperature was 24 ±1 °C. After 96 hr exposure at the 100 mg/L group, no death was observed. Therefore the 96-hr LC50 was determined to be greater than 100 mg/L. The other studies support this result.

Key value for chemical safety assessment

Additional information