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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1976
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study without detailed documentation

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1976
Report Date:
1976

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
Version / remarks:
reliability scoring based on 2002 guideline
Deviations:
yes
Remarks:
Source, purity, chemical characteristics of test substance, temperature, light cycle, and humidity not provided; grading scale for irritation not reported
GLP compliance:
no
Remarks:
study pre-dates GLP requirements

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 1,2,4-triazole
- Analytical purity: reported as technically pure

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White
Details on test animals or tissues and environmental conditions:
TEST ANIMALS
- Source: from the breeder Winkelmann (Borchen)
- Age at study initiation: no data
- Weight at study initiation: 3-4kg
- Fasting period before study: no data
- Housing: singly in standard rabbit cages
- Diet (e.g. ad libitum): Rabbit food 222 (Hoeing co.), ad libitum
- Water (e.g. ad libitum): not precised, ad libitum
- Acclimation period: no data

ENVIRONMENTAL CONDITIONS: no data

Test system

Vehicle:
unchanged (no vehicle)
Controls:
not required
Amount / concentration applied:
Test article was instilled into the conjuctival sac of the left eye of 2 rabbits at a dose of 50 mg/animal.
Duration of treatment / exposure:
Single administration
Observation period (in vivo):
7 days
Number of animals or in vitro replicates:
2

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
mean
Remarks:
2 rabbits
Time point:
other: 24-48h
Score:
0.5
Max. score:
4
Reversibility:
fully reversible
Remarks on result:
other: (1 rabbit)
Irritation parameter:
iris score
Basis:
mean
Remarks:
2 rabbits
Time point:
other: 24-48 h
Score:
0.5
Max. score:
2
Reversibility:
fully reversible
Remarks on result:
other: (1 rabbit)
Irritation parameter:
conjunctivae score
Remarks:
(redness)
Basis:
mean
Remarks:
2 rabbits
Time point:
other: 24-48h
Score:
2.5
Max. score:
4
Reversibility:
fully reversible
Irritation parameter:
chemosis score
Basis:
mean
Remarks:
2 rabbits
Time point:
other: 24-48 h
Score:
1
Max. score:
4
Reversibility:
fully reversible
Remarks on result:
other: (1 rabbit)
Irritant / corrosive response data:
One hour after application intense reddening and a very intense swelling of the conjectivae of the treated eyes had developed. 24 hours after application intense reddening and moderate swelling of the conjuctivae was still present. In one animal, there was still slight redness and swelling of the conjuctivae 5 days after application, while it was normal in the other animal. The conjuctivae of both animals were normal after 7 days. During the first and second days after application, a slight, dispersed, diffuse opacity of the cornea was observed. The iris was slightly reddened and swollen.

Applicant's summary and conclusion

Interpretation of results:
other: Category 2 : Causes serious eye irritation.
Remarks:
Criteria used for interpretation of results: other: CLP (EC1272/2008)
Conclusions:
The authors concluded that 1,2,4-triazole has a irritant effect on the mucous membrane.
Executive summary:

A non GLP comparable Guideline 405 (Acute Eyes Irritation / Corrosion) test was carried out with 1,2,4 -triazole.

Undiluted test article was instilled into the conjuctival sac of the left eye of 2 rabbits at a dose of 50 mg/animal.

One hour after application intense reddening and a very intense swelling of the conjectivae of the treated eyes had developed. 24 hours after application intense reddening and moderate swelling of the conjuctivae was still present. In one animal, there was still slight redness and swelling of the conjuctivae 5 days after application, while it was normal in the other animal. The conjuctivae of both animals were normal after 7 days. During the first and second days after application, a slight, dispersed, diffuse opacity of the cornea was observed. The iris was slightly reddened and swollen.

Under the experimental conditions, the test substance is irritant and is classified in category 2 according to regulation EC no.1272/2008 (CLP).