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Toxicological information

Acute Toxicity: other routes

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Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Well documented study report which meets basic scientific principles

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1962
Report Date:
1962

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
other: BASF Test following internal SOP
GLP compliance:
no
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Isobutylacrylate stabilised with 0.006 % Hydrochinonmonomethylether

Test animals

Species:
mouse
Strain:
not specified
Sex:
male/female

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
other: 20%, 8%, 4%, 2% aqueous emulsion with traganth
Details on exposure:
single intraperitoneal injection
Doses:
200, 250, 320, 500, 800, 1600 mg/kg bw
No. of animals per sex per dose:
10 animals; 200 mg/kg bw dosing group only 5 animals
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations: daily on working days
- Frequency of weighing: prior to dosing
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
ca. 400 mg/kg bw
Mortality:
200 mg/kg dosing group: 1/5 (48 hours); 1/5 in total
250 mg/kg dosing group: 3/10 (7 days); 4/10 in total
320 mg/kg dosing group: 1/10 (48 hours); 4/10 in total
400 mg/kg dosing group: 1/10 (48 hours); 4/10 (7 days); 4/10 in total
500 mg/kg dosing group: 1/10 (48 hours); 6/10 (7 days); 6/10 in total
800 mg/kg dosing group: 6/10 (24 hours); 7/10 (48 hours); 10/10 (7 days); 10/10 in total
1600 mg/kg dosing group: 9/10 (24 hours); 9/10 (48 hours); 9/10 (7 days); [14 day observation missing]
Clinical signs:
narcosis, staggering, apathy, accelerated respiration; lateral position, late deaths;
following days: apathy, ruffled coat; symptomes decreased till the end of the observation period
Gross pathology:
survivers: organs without findings;
deceased animals: enteritis in one animal; irritation of the peritoneum with some local adhesions in 1 animal

Applicant's summary and conclusion