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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well documented study report which meets basic scientific principles.

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1968
Report Date:
1968
Reference Type:
study report
Title:
Unnamed
Year:
1967
Reference Type:
study report
Title:
Unnamed
Year:
1991

Materials and methods

Principles of method if other than guideline:
Six rats per dose group were exposed to isobutyl acrylate vapours at metered concentrations (not checked analytically) of 1000, 2000 and 4000 ppm (corresponding to approx. 5.2, 10.5 and 21 mg/L) in a 9 liter glass exposure chamber for 4 h. The animals were observed for 14 days and the LC50 was calculated by the moving average method based on a 14-day observation period.
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Isobutyl acrylate
-Supplier: Union Carbide
- Physical state: liquid
- Analytical purity: no data
- Lot/batch No.: 02497

Test animals

Species:
rat
Strain:
not specified
Sex:
not specified
Details on test animals and environmental conditions:
TEST ANIMALS
- Fasting period before study: none
- Diet (e.g. ad libitum): Rockland diets
- Water: ad libitum

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
other: unchanged (no vehicle)
Details on inhalation exposure:
GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
Vapours at metered concentrations, not checked analytically, were generated by feeding the liquid at a constant rate down the inside of a spirally corrugated surface of a minimally heated one inch Pyrex tube, through which metered air was passed. Resultant vapours were delivered to rats in a 9-liter glass exposure chamber.
Analytical verification of test atmosphere concentrations:
no
Duration of exposure:
4 h
Concentrations:
1000, 2000, 4000 ppm (corresponding to approximately 5.2, 10.5, 21 mg/L) Calculation of concentrations (mg/L) based on Derelanko MJ (2000). Toxicologist's Pocket Handbook, CRC Press, conversion table, p. 57.
No. of animals per sex per dose:
6
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes/no
- Other examinations performed: clinical signs, gross pathology

Results and discussion

Effect levels
Sex:
not specified
Dose descriptor:
LC50
Effect level:
ca. 10.5 mg/L air
95% CL:
7.4 - 14.8
Exp. duration:
4 h
Mortality:
No mortality was observed in 1000 ppm dose group; 3/6 animals died in 2000 ppm dose group at days 4,5,6 after exposure; all animals died in 4000 ppm dose group on the day of exposure.
Clinical signs:
other: 1000 ppm dose group: extremities irritated at 3 h of exposure; 2000 ppm dose group: eyes and noses irritated at 1 h of exposure, and gasping at 3 h of exposure; 4000 ppm dose group: Rubbing noses and keeping eyes closed immmediately, hyperactive at 30 min
Gross pathology:
Abnormalities observed through necropsy included capillary injection on all animals at the highest dose group. Consolidation of the lungs in 3 animals in the middle dose group. Nothing remarkable in the surviving animals.

Any other information on results incl. tables

Original values reported: LC50 / 4 hrs = 2000 (1419 - 2825) ppm which corresponds to ca. 10.5 (7.4-14.8) mg/L.

Applicant's summary and conclusion

Interpretation of results:
harmful
Remarks:
Migrated information Criteria used for interpretation of results: EU