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Toxicological information

Basic toxicokinetics

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Administrative data

Endpoint:
basic toxicokinetics in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
25 Jan 2001 to 30 Jan 2001
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable study report which meets basic scientific principles

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2001
Report Date:
2001

Materials and methods

Objective of study:
metabolism
Principles of method if other than guideline:
Isobutyl acrylate was tested for relative rates of hydrolysis by mammalian esterase.
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Isobutyl acrylate
- Analytical purity: 99.88 %
- Lot/batch No.: S0308-01-GA
Radiolabelling:
no

Administration / exposure

Doses / concentrations
Remarks:
Doses / Concentrations:
0.2, 0.5 and 2 mM

Results and discussion

Toxicokinetic / pharmacokinetic studies

Toxicokinetic parameters
Toxicokinetic parameters:
other: Isobutyl acrylate was rapidly hydrolyzed at rates ranging between 45 - 47 µmoles/min/mg protein (3 - 27 %) after 2 minutes and 38 - 49 µmoles/min/mg protein (7 - 57 %) after 5 minutes of incubation at 37°C.

Metabolite characterisation studies

Metabolites identified:
no

Any other information on results incl. tables

The % conversion results of isobutyl acrylate (an average of 3 determinations)

Concentration (mM)

% Conversion / Rate*

At 2 min.

At 5 min.

2.00

2.83 / 47.17

7.28 / 48.53

0.50

14.88 / 62.00

34.17 / 56.95

0.20

27.07 / 45.12

57.44 / 38.29

* in μmoles / min / mg protein

Isobutyl acrylate was rapidly hydrolyzed at rates ranging between 45 - 47 µmoles/min/mg protein (3 - 27 %) after 2 minutes and 38 - 49 µmoles/min/mg protein (7 - 57 %) after 5 minutes of incubation at 37°C.

Applicant's summary and conclusion