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Short-term toxicity to fish

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Key value for chemical safety assessment

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In a key semi-static 96-hour acute rainbow trout (Oncorhynchus mykiss) test (OECD 203; KS=1),10 animals/dose were exposed to water accommodated fractions (WAFs) of Intermediate fuel oils 30-380 (CAS # 68476-33-5) at loading rates of 0, 2.0, 5.1, 13, 32, and 79 mg/L. The LL50 was 79 mg/L (EMBSI, 2008a). 

In six reliable supporting semi-static 96-hour acute rainbow trout (Oncorhynchus mykiss) studies using WAF methodology, the LL50 values of other heavy fuel oil samples ranged from >94 to >1000 mg/L (EMBSI, 2008b,c,d; Shell, 1997a,b).