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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

The acute toxicity of aluminum, benzoate C16-18-fatty acids complexes to the freshwater fish rainbow trout (Oncorhynchus mykiss)  showed no effects at a water accommodated fraction nominal loading rate of 100 mg/L. Therefore, the 96 hour LL50 is determined to be > 100 mg/L (WAF).

Key value for chemical safety assessment

Additional information

The acute toxicity to rainbow trout (Oncorhynchus mykiss) was determined in GLP-compliant, semi-static limit test (Harlan 2013) following OECD guideline 203. As no effects were observed in the Daphnia and algae toxicity tests or the preliminary range finding test for acute toxicity to fish, the definitive toxicity to fish test was conducted as a limit test at a nominal loading rate of 100 mg/L alongside a blank control. Seven fish were exposed to a water accommodated fraction (WAF) of aluminum, benzoate C16-18-fatty acids complexes for 96 hours in standard ecotoxicity media adjusted to a hardness of approximately 150 mg/L CaCO3. Observations of mortalities or abnormalities of behaviour or appearance of the fish were made at 3, 6, 24, 48, 72 and 96 hours and the test solutions analysed for aluminium content and total organic carbon at test initiation and termination. As no toxicity effects were observed, the 96 hour LL50 is determined to be > 100 mg/L (WAF).