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EC number: 915-741-3 | CAS number: 25618-55-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
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- Auto flammability
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- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
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- Nanomaterial surface chemistry
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- Endpoint summary
- Stability
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- Environmental data
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
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- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Carcinogenicity
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- Specific investigations
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- Additional toxicological data

Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 25 June - 09 July 2009
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study was performed according to OECD and EC guidelines and according to GLP principles.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 1-(1,3-dihydroxypropoxy)propane-1,3-diol; 3-{3-[3-(2,3-dihydroxypropoxy)-2-hydroxypropoxy]-2-hydroxypropoxy}propane-1,2-diol; nonane-1,2,4,5,6,8,9-heptol
- EC Number:
- 915-741-3
- Cas Number:
- 25618-55-7
- Molecular formula:
- not applicable - multi-constituent substance
- IUPAC Name:
- 1-(1,3-dihydroxypropoxy)propane-1,3-diol; 3-{3-[3-(2,3-dihydroxypropoxy)-2-hydroxypropoxy]-2-hydroxypropoxy}propane-1,2-diol; nonane-1,2,4,5,6,8,9-heptol
- Reference substance name:
- Polyglycerol-3
- IUPAC Name:
- Polyglycerol-3
- Details on test material:
- - Name of test material (as cited in study report): Polyglycerol-3
- Substance type: organic liquid
- Physical state: Colourless to pale yellow viscous liquid
- Analytical purity:
27.9% Diglycerol
46.0% Triglycerol
17.9% Tetraglycerol
5.6% Pentaglycerol
2.6% Hexaglycerol and higher
- Purity test date: no data
- Lot/batch No.: RBA081008A
- Expiration date of the lot/batch: 31 May 2010
- Stability under test conditions: stabile
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- not required
Test solutions
- Vehicle:
- no
- Details on test solutions:
- A stock solution of 0.5 g/l was prepared by adding 0.1257 g test substance to 250 ml of Milli-RO water (tap water purified by reverse osmosis; Millipore Corp., Bedford, Mass., USA). Thorough mixing (15 minutes) was used to accelerate dissolution and to ensure homogeneity. Volumes of the stock solution corresponding to the test concentration were then added to the test media. A concentration of 100 mg/l was tested in duplicate.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Source: Municipal sewage treatment plant: ‘Waterschap de Maaskant', 's-Hertogenbosch, The Netherlands, receiving predominantly domestic sewage.
- Preparation of inoculum for exposure: The sludge was coarsely sieved, washed and diluted with ISO-medium. A small amount of the sludge was weighed and dried overnight at ca. 105°C to determine the amount of suspended solids (4.0 g/l of sludge, as used for the test). The pH was 7.8 on the day of testing. The batch of sludge was used one day after collection; therefore 50 ml of synthetic sewage feed was added per litre of activated sludge at the end of the collection day. The sludge was kept aerated at test temperature until use.
- Initial biomass concentration: 4.0 g/l (of suspended solids)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Post exposure observation period:
- none
Test conditions
- Test temperature:
- 18°C.
- pH:
- 8.2-8.3
- Dissolved oxygen:
- 8.6-8.9
- Nominal and measured concentrations:
- nominal: 100 mg/l
- Details on test conditions:
- The synthetic sewage feed (16 ml), activated sludge (200 ml) and an adequate amount of the test substance stock were mixed and made up to 500 ml with Milli-RO water in a 1 litre bottle. The mixture was aerated during the contact time, using a pipette as an aeration device.
After the 3-hour contact time, a well mixed sample of the contents was poured into a 300 ml oxygen bottle, and the flask was sealed with an oxygen electrode connected to a recorder, forcing the air out of the vessel. Oxygen consumption was measured and recorded for approximately 10 minutes. During measurement, the sample was not aerated but continuously stirred on a magnetic stirrer.
The pH and temperature were determined in the remaining part of the reaction mixture.
This procedure was repeated for the duplicate concentration. Two controls without test substance were tested in each test series, one at the start and one at the end.
Medium: Adjusted ISO medium, formulated using RO-water (tap-water purified by reverse osmosis; GEON Waterbehandeling, Berkel-Enschot, The Netherlands) - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- No significant inhibition of respiration rate of the sludge was recorded at 100 mg Polyglycerol-3 per litre and therefore no further testing was needed. Hence, the EC50 of Polyglycerol-3 exceeded 100 mg/l (based on nominal concentration).
- Results with reference substance (positive control):
- Ec50 = 7.8 mg/l
- Reported statistics and error estimates:
- For Polyglycerol-3 no EC50 could be calculated because the test substance proved to be non-toxic (EC50 > 100 mg/l).
Any other information on results incl. tables
none
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of this present test, Polyglycerol-3 was not toxic to waste water (activated sludge) bacteria at a nominal concentration of 100 mg/l.
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