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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
27 August 1999 - 03 September 1999
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
Qualifier:
according to
Guideline:
EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
Deviations:
no
Qualifier:
according to
Guideline:
ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)- Eluate (conc.): 100 mg/L- Controls: Potassium dichromate- Concentration of vehicle in test medium (stock solution and final test solution): The test substance was stirred in M4 medium for about 48 hours at 20 ± 2 0C. Undissolved test substance was removed by centrifugation (approximately 20 min at about 17700 G). The nominal concentration of the eluate was 100 mg/L. By diluting this eluate with M4 medium the following nominal concentrations were prepared: 0, 100, 50, 25, 12,5 mg/L
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM- Common name: Water flea- Source: Laboratory of Ecology of BASF AG in Ludwigshafen- Age at study initiation: 2-24 h- Feeding during test: None
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
2.42 mmol/L
Test temperature:
20.4 °C
pH:
8.1
Dissolved oxygen:
7.7-8.3
Nominal and measured concentrations:
Nominal concentrations: 12.5, 25, 50 and 100 mg/L
Details on test conditions:
TEST SYSTEM- Test vessel: - Material, size, headspace, fill volume: Test tubes (glass) with flat bottom (nominal volume 20 mL) - Aeration: M4 medium aerated for 24 h until saturation with O2- No. of organisms per vessel: Five- No. of vessels per concentration (replicates): Four- No. of vessels per control (replicates): Four- Biomass loading rate: 0.5 animals/mLTEST MEDIUM: M4 medium- Source/preparation of dilution water: Synthetic fresh water (ultrapure and demineralized) was used for culture and test purposes- Conductivity: 615 µS/cm- Ca/mg ratio: 4:1- Alkalinity: 0.80-1.00 mmol/LOTHER TEST CONDITIONS- Photoperiod: 16 h artificial white light / 8 h night rhythm- Light intensity: 2-7 µE/m2.s at wavelength of 400-700 nmEFFECT PARAMETERS MEASURED (with observation intervals if applicable): Swimming ability of the test animals recorded after 0, 24 and 48 h
Reference substance (positive control):
yes
Remarks:
Potassium dichromate
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
EC50 (24 h) = 0.68 mg/L
Reported statistics and error estimates:
Test results allowed no statistical evaluation of the data.
Validity criteria fulfilled:
yes
Remarks:
In the control, Immobilization was ≤ 10 % and no daphnids were captured in a surface film; O2-concentration was > 3 mg/L in all tested replicates; pH-value in the test solutions did not vary within more than one unit.
Conclusions:
Under the test conditions, the 48 h EC50 of the test substance to Daphnia was > 100 mg/L (nominal). The 48 h EC0 was greater than or equal to 100 mg/L (nominal) and the 48 h EC100 was > 100 mg/L (nominal).
Executive summary:

A study was performed to assess the acute toxicity of the test substance to Daphnia magna according to OECD Guideline 202, EPA OPPTS 850.1010 and ISO 634115.

Daphnia magna (4 replicates of 5 animals/dose) were exposed to nominal concentratrions of the test substance (0, 12.5, 25, 50 and 100 mg/L) in M4 medium for 48 h in static test conditions at 20°C. Animals were observed for immobilisation after 0, 24 and 48 h. Potassium chromate was used as positive control. The EC50 (24 h) of the control substance was estimated to be 0.68 mg/L.

The test was performed without concentration analysis because the substance is rapidly hydrolysed.

Under the test conditions, the 48 h EC50 of the test substance to Daphnia was > 100 mg/L (nominal). The 48 h EC0 was greater than or equal to 100 mg/L (nominal) and the 48 h EC100 was > 100 mg/L (nominal).

Description of key information

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
100 mg/L

Additional information

A study was performed to assess the acute toxicity of the test substance to Daphnia magna according to OECD Guideline 202, EPA OPPTS 850.1010 and ISO 634115.No analytical determination of dose levels was carried out as the substance hydrolyses rapidly. Results were presented based on nominal concentrations.The 48 h EC50 was > 100 mg/L (nominal). The 48 h EC0 was ≥ 100 mg/L (nominal) and the 48 h EC100 was > 100 mg/L (nominal).