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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
06 September 1999 - 10 September 1999
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
yes
Remarks:
stability of the test substance not determined analytically (this deviation was considered not to adversely affect the validity and overall results)
Qualifier:
according to
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
yes
Details on test solutions:
- The test substance was unstable in water with a degradation rate of about 90 % within 24 hours. lt was therefore technically not possible to test the substance itself. lt was decided to test the products of hydrolysis without analytical identification and monitoring, since this was not possible with justifiable expense.- Preparation of test solution: Test substance (1,000 mg) was added to 10 L test water and the mixture was homogenized using an ultra-turrax stirrer. The test concentrations were prepared 2 days before the test organisms were introduced into the test vessels, so that a nearly complete hydrolysis could take place before the insertion of the test organisms
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM- Common name: Zebra fish- Source: Zierfisch-Center Kloeckner, Ludwigshafen/Rhein, Germany- Length at study initiation (length definition, mean, range and SD): 3.4 (3.1-3.7) cm- Weight at study initiation (mean and range, SD): 0.33 (0.25-0.46) gACCLIMATION- Acclimation period: 14 days- Acclimation conditions: Fish were kept in a flow-through tank in tap water, which was not chlorinated, passed through a charcoal filter and aerated with oil-free air- Type and amount of food: 'TETRA MIN' standard feed for aquarium fish (TETRA - WERKE, MELLE, Germany), ad libitum- Feeding frequency: Fed on workdays- Health during acclimation (any mortality observed): No mortality
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
2.5 mmol/L, equivalent to 250 mg/L CaCO3
Test temperature:
23 °C
pH:
8.3-8.5
Dissolved oxygen:
8.0-8.6 mg/L
Nominal and measured concentrations:
Nominal concentrations: 0 and 100 mg/L
Details on test conditions:
TEST SYSTEM- Test vessel: - Material, size, headspace, fill volume: All-glass aquaria (30 X 22 X 24 cm), 10 L; one aquarium for each test group - Aeration: Slight- No. of organisms per vessel: 10- Biomass loading rate: 0.3 g/LTEST MEDIUM / WATER PARAMETERS- Source/preparation of dilution water: Municipal water (City of Frankenthal), not chlorinated, passed through a charcoal filter and aerated- Acid capacity: 5.5 mmol/L- pH: 8.0-8.6- Ca/mg ratio: 9:1- Intervals of water quality measurement: RegularOTHER TEST CONDITIONS- Photoperiod: 16 h light/ 8 h darkEFFECT PARAMETERS MEASURED (with observation intervals if applicable) : - Symptoms and mortality: Recorded at 1, 4, 24, 48, 72 and 96 hours after study initiation. Water parameter (pH value, oxygen content and water temperature) were also measured at these points of time.TEST CONCENTRATIONS- Range finding study - Results used to determine the conditions for the definitive study: LC50 (96 h) > 100 mg/L
Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Behavioural abnormalities: No- Mortality of control: No
Reported statistics and error estimates:
Median lethal concentration (LC50) after 1, 4, 24, 48, 72 and 96 hours, based on the nominal concentration was calculated using probit analysis.
Validity criteria fulfilled:
yes
Remarks:
No mortality in control; constant condition; dissolved O2 concentration > 60 % of saturation value; analytical verification of the concentration of the substance during the test was technically not possible.
Conclusions:
The 96 h LC50 of the test substance to Zebra fish was determined to be > 100 mg/L (nominal). The 96 h NOEC was 100 mg/L (nominal).
Executive summary:

A study was conducted to determine the acute toxicity (LC50) of amine synergist to Zebra fish according to OECD Guideline 203 and EU Method C.1.

The test substance is instable in water with a degradation rate of about 90% within 24 h. lt was therefore technically not possible to test the substance itself. lt was decided to test the products of hydrolysis without analytical identification and monitoring. The test concentrations were prepared 2 days before the test organisms were introduced into the test vessels, so that a nearly complete hydrolysis could take place before the insertion of the test organisms.

A group of twenty Zebra fish (10/dose) were exposed to the test substance at 0 and 100 mg/L in static conditions. Mortality and behavioural abnormalities were recorded at 1, 4, 24, 48, 72 and 96 h. No mortalities or behavioural effects were noted at 0 and 100 mg/L.

The 96 h LC50 of the test substance to Zebra fish was determined to be > 100 mg/L (nominal). The 96 h NOEC was 100 mg/L (nominal).

Description of key information

Key value for chemical safety assessment

LC50 for freshwater fish:
100 mg/L

Additional information

A study was conducted to determine the acute toxicity of amine synergist to Zebra fish (Brachydanio rerio) according to OECD Guideline 203 and EU Method C.1. No analytical determination of dose levels was carried out as the substance hydrolyses rapidly. Results were presented based on nominal concentrations. No mortality or behavioural effects were noted at 100 mg/L. The 96 h LC50 was therefore > 100 mg/L (nominal). The 96 h NOEC was equal to 100 mg/L (nominal) (Zok, 2000).