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EC number: 601-101-8 | CAS number: 111497-86-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 06 September 1999 - 10 September 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- yes
- Remarks:
- stability of the test substance not determined analytically (this deviation was considered not to adversely affect the validity and overall results)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
- - The test substance was unstable in water with a degradation rate of about 90 % within 24 hours. lt was therefore technically not possible to test the substance itself. lt was decided to test the products of hydrolysis without analytical identification and monitoring, since this was not possible with justifiable expense.- Preparation of test solution: Test substance (1,000 mg) was added to 10 L test water and the mixture was homogenized using an ultra-turrax stirrer. The test concentrations were prepared 2 days before the test organisms were introduced into the test vessels, so that a nearly complete hydrolysis could take place before the insertion of the test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM- Common name: Zebra fish- Source: Zierfisch-Center Kloeckner, Ludwigshafen/Rhein, Germany- Length at study initiation (length definition, mean, range and SD): 3.4 (3.1-3.7) cm- Weight at study initiation (mean and range, SD): 0.33 (0.25-0.46) gACCLIMATION- Acclimation period: 14 days- Acclimation conditions: Fish were kept in a flow-through tank in tap water, which was not chlorinated, passed through a charcoal filter and aerated with oil-free air- Type and amount of food: 'TETRA MIN' standard feed for aquarium fish (TETRA - WERKE, MELLE, Germany), ad libitum- Feeding frequency: Fed on workdays- Health during acclimation (any mortality observed): No mortality
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 2.5 mmol/L, equivalent to 250 mg/L CaCO3
- Test temperature:
- 23 °C
- pH:
- 8.3-8.5
- Dissolved oxygen:
- 8.0-8.6 mg/L
- Nominal and measured concentrations:
- Nominal concentrations: 0 and 100 mg/L
- Details on test conditions:
- TEST SYSTEM- Test vessel: - Material, size, headspace, fill volume: All-glass aquaria (30 X 22 X 24 cm), 10 L; one aquarium for each test group - Aeration: Slight- No. of organisms per vessel: 10- Biomass loading rate: 0.3 g/LTEST MEDIUM / WATER PARAMETERS- Source/preparation of dilution water: Municipal water (City of Frankenthal), not chlorinated, passed through a charcoal filter and aerated- Acid capacity: 5.5 mmol/L- pH: 8.0-8.6- Ca/mg ratio: 9:1- Intervals of water quality measurement: RegularOTHER TEST CONDITIONS- Photoperiod: 16 h light/ 8 h darkEFFECT PARAMETERS MEASURED (with observation intervals if applicable) : - Symptoms and mortality: Recorded at 1, 4, 24, 48, 72 and 96 hours after study initiation. Water parameter (pH value, oxygen content and water temperature) were also measured at these points of time.TEST CONCENTRATIONS- Range finding study - Results used to determine the conditions for the definitive study: LC50 (96 h) > 100 mg/L
- Reference substance (positive control):
- not specified
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: No- Mortality of control: No
- Reported statistics and error estimates:
- Median lethal concentration (LC50) after 1, 4, 24, 48, 72 and 96 hours, based on the nominal concentration was calculated using probit analysis.
- Validity criteria fulfilled:
- yes
- Remarks:
- No mortality in control; constant condition; dissolved O2 concentration > 60 % of saturation value; analytical verification of the concentration of the substance during the test was technically not possible.
- Conclusions:
- The 96 h LC50 of the test substance to Zebra fish was determined to be > 100 mg/L (nominal). The 96 h NOEC was 100 mg/L (nominal).
- Executive summary:
A study was conducted to determine the acute toxicity (LC50) of amine synergist to Zebra fish according to OECD Guideline 203 and EU Method C.1.
The test substance is instable in water with a degradation rate of about 90% within 24 h. lt was therefore technically not possible to test the substance itself. lt was decided to test the products of hydrolysis without analytical identification and monitoring. The test concentrations were prepared 2 days before the test organisms were introduced into the test vessels, so that a nearly complete hydrolysis could take place before the insertion of the test organisms.
A group of twenty Zebra fish (10/dose) were exposed to the test substance at 0 and 100 mg/L in static conditions. Mortality and behavioural abnormalities were recorded at 1, 4, 24, 48, 72 and 96 h. No mortalities or behavioural effects were noted at 0 and 100 mg/L.
The 96 h LC50 of the test substance to Zebra fish was determined to be > 100 mg/L (nominal). The 96 h NOEC was 100 mg/L (nominal).
Reference
Description of key information
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
A study was conducted to determine the acute toxicity of amine synergist to Zebra fish (Brachydanio rerio) according to OECD Guideline 203 and EU Method C.1. No analytical determination of dose levels was carried out as the substance hydrolyses rapidly. Results were presented based on nominal concentrations. No mortality or behavioural effects were noted at 100 mg/L. The 96 h LC50 was therefore > 100 mg/L (nominal). The 96 h NOEC was equal to 100 mg/L (nominal) (Zok, 2000).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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