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Environmental fate & pathways

Adsorption / desorption

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Reference
Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Study period:
19 March 2010 - 08 April 2010
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
Deviations:
no
GLP compliance:
yes
Type of method:
HPLC estimation method
Media:
other: based on soil-adsorption-reference data
Test temperature:
35°C +/- 1°C
Details on study design: HPLC method:
Analytical conditionsInstrument: Alliance Separation Module 2695 (Waters, Milford, MA, USA)Detector: Dual λ Absorbance Detector 2487 (Waters) Column: Hypersil BDS-CN, 150 mm x 4.6 mm i.d., dp = 5 µm (Thermo Fisher Scientific, Waltham, MA, USA)Column temperature: 35°C +/- 1°CMobile phase: 30/70 (v/v) methanol/waterFlow: 1 ml/minInjection volume: 10 µlUV detection: 210 nmPreparation of the solutionsAll solutions containing the test substance were protected from light. Solution of the unretained compoundA 1.07 g/l stock solution of thiourea (>99%, Acros Organics, Geel, Belgium) in methanol was used. The stock solution was 100-times diluted in methanol and analysed. The thiourea blank solution was methanol.Reference substance solutionsStock solutions of the reference substances at concentrations of approximately 1 g/l in methanol were used. The stock solutions were 100-times diluted in methanol and analysed. The blank solution for the mixture of reference substances was methanol. Reference substances used:Acetanilide, Atrazine, Monuron, 2,5-Dichloroaniline, Benzoic acid phenylester, Fenthion, PhenanthreneTest solutionA 18.6 g/l stock solution of the test substance was prepared in methanol. The stock solution was diluted in methanol to obtain a test solution at a concentration of 497 mg/l. The test substance blank solution was methanol.Note: with preliminary research it was observed that the test substance was unstable in 30/70 (v/v) methanol/water. According to this, pure methanol was selected as end solution of the reference and test substance solutions. It was found that the methanol end solution had no effect on the capacity factors and, therefore, no effect on the Koc values of the test substance.InjectionsThe reference substance and test substance solutions were injected in duplicate. Blank solutions were analysed by single injection.
Key result
Type:
log Koc
Value:
>= 0.42
Temp.:
35 °C
Remarks on result:
other: Compound I, 14% peak area
Type:
log Koc
Value:
1.17
Temp.:
35 °C
Remarks on result:
other: Compound II, 62% peak area
Type:
log Koc
Value:
1.51
Temp.:
35 °C
Remarks on result:
other: Compound III, 5% peak area
Type:
log Koc
Value:
2.12
Temp.:
35 °C
Remarks on result:
other: Compound IV, 17% peak area
Type:
log Koc
Value:
2.49
Temp.:
35 °C
Remarks on result:
other: Compound V, 2.3% peak area
Details on results (HPLC method):
The log Koc values at neutral pH of Ebecryl®P116 radiation curing resins based on soil-adsorption-reference data were: Compound I: 0.42 (14% peak area)Compound II: 1.17 (62% peak area)CompoundIII: 1.51 (5.0% peak area)Compound IV: 2.12 (17% peak area)Compound V: 2.49 (2.3% peak area) In summary, the log Koc of the test substance, Ebecryl®P116 radiation curing resins was between 0.42 and 2.49.
Validity criteria fulfilled:
yes
Conclusions:
Under the test conditions, the log Koc of the test substance was established to range from 0.42 to 2.49.
Executive summary:

A study was conducted to determine the adsorption of the test substance according to OECD Guideline 121 and EU Method C.19. The HPLC method was used for determination of adsorption coefficient of the test substance.

The test substance was unstable when diluted in methanol/water, therefore only methanol was employed as a diluent.

Under the test conditions, the log Koc of the test substance was established to range from 0.42 to 2.49. The log Koc of the two major peaks (in % peak area) were 1.17 (62% peak area) and 2.12 (17% peak area).

Description of key information

 A study was conducted to determine the adsorption/desorption of amine synergist according to OECD Guideline 121 and EU Method C.19 (HPLC estimation).The test substance was unstable when diluted in methanol/water, therefore only methanol was used as a diluent.The log Koc of the various peaks determined by HPLC was established to range from 0.42 to 2.49. The log Koc of the two major peaks (in % peak area) were 1.17 (62% peak area) and 2.12 (17% peak area) (Oudhoff, 2010). The data suggest low binding to soil. 

Key value for chemical safety assessment

Koc at 20 °C:
310

Additional information

.

[LogKoc: 2.49]