Reaction mass of pentadecylamine and tridecylamine and 2-Methyldodecylamine and 2-Methyltetradecylamine

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

10.06.1997 - 13.08.1997

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Version / remarks:

Adopted: 4 April 1984

GLP compliance:

yes

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
- Water solubility (under test conditions): 500 mg/L

Analytical monitoring:

no

Details on sampling:

- Concentrations: 6.3, 12.5, 25.0, 50.0, 100 mg/L

Test organisms (species):

activated sludge, domestic

Details on inoculum:

- Source: municipal sewage plant Sindlingen (Frankfurt/ Main)
- Suspension: 450 ml/L, after centrifugation 30.03 g/ sedimented sludge (dry mass: 3.56g/L)
- Preparation of inoculum for exposure: activated sludge was 3x centrifuged, resuspended in water containing 0.9% NaCl
- Pretreatment: not adapted
- Initial biomass concentration: 1.6 g/L (dry mass, final concentration in test volume)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Remarks on exposure duration:

Incubation time with TS

Hardness:

no data

Test temperature:

19.3°C - 20.4°C

pH:

7.0 - 7.8 (after incubation)

Dissolved oxygen:

6.0 mg/L - 8.3 mg/L at test start

Salinity:

no data

Nominal and measured concentrations:

6.3, 12.5, 25.0, 50.0, 100 mg/L (nominal)

Details on test conditions:

TEST SYSTEM
- Material, size, headspace, fill volume: 200 mL
- Aeration: 50 L/h during incubation
- No. of vessels per concentration (replicates): two
- No. of vessels per control (replicates): three

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Reference substance (positive control):

yes

Remarks:

3,5-dichlorphenol (5, 20, and 30 mg/L)

Duration:

3 h

Dose descriptor:

EC0

Effect conc.:

> 6.3 - < 12.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other: see report table 2 (page 8)

Duration:

3 h

Dose descriptor:

other: EC20

Effect conc.:

16 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Key result

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

33 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Duration:

3 h

Dose descriptor:

other: EC80

Effect conc.:

66 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Results with reference substance (positive control):

- Results with reference substance valid? yes
- EC50: 12 mg/L (C.I.: 8 mg/L - 15 mg/L, P=95 %)

Reported statistics and error estimates:

Calculation of EC20, EC50, and EC80: Probit-Analysis (Finney 1971), Confidence interval was uncalculable

Validity criteria fulfilled:

yes

Conclusions:

In an activated sludge, respiration inhibition test the EC50 (3 hours) was calculated to be between > 10 mg/L and 100 mg/L.

Executive summary:

The effect of n/i-C13/C15 amine on the respiration rate of activated sludge was assessed in a GLP study according to OECD 209.

Samples of activated sludge were exposed to the test substance at nominal concentrations of 6.3, 12.5, 25.0, 50.0 and 100.0 mg/L for 3 hours. Their rates of oxygen consumption were determined and compared with those of controles, which were established at the beginning and end of the culture series.

The reference inhibitor 3,5 -dichlorophenol (3,5 -DCP) was employed in the test at 5, 20 and 30 mg/L, as a positive control. The three-hour 50% effect concentration (EC50) for 3,5 -DCP was calculated to be 12 mg/L (95% confidence limits 8 - 15 mg/L). These results show that the rest was valid and that the sample of activated sludge employed was sentitve to inhibition.

n/i-C13/C15 amine had an inhibitory effect on the respiration rate of activated sludge, resulting in an EC 50 value of > 10 mg/L - 100 mg/L.

Description of key information

In an activated sludge, respiration inhibition test the EC50 (3 hours) was calculated to be between > 10 mg/L and 100 mg/L.

Additional information

The effect of n/i-C13/C15 amine on the respiration rate of activated sludge was assessed in a GLP study according to OECD 209.

Samples of activated sludge were exposed to the test substance at nominal concentrations of 6.3, 12.5, 25.0, 50.0 and 100.0 mg/L for 3 hours. Their rates of oxygen consumption were determined and compared with those of controles, which were established at the beginning and end of the culture series.

The reference inhibitor 3,5 -dichlorophenol (3,5 -DCP) was employed in the test at 5, 20 and 30 mg/L, as a positive control. The three-hour 50% effect concentration (EC50) for 3,5 -DCP was calculated to be 12 mg/L (95% confidence limits 8 - 15 mg/L). These results show that the rest was valid and that the sample of activated sludge employed was sentitve to inhibition.

n/i-C13/C15 amine had an inhibitory effect on the respiration rate of activated sludge, resulting in an EC 50 value of > 10 mg/L - 100 mg/L.