2,2'-dimorpholinyldiethyl ether

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

not indicated

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Remarks:

Non-GLP study performed according to a method similar to the Inhalation Hazard Test in the annex of OECD Guideline 403. Rats were exposed 8 h instead of 7 h. Details on the method are partly lacking and can thus not be compared with the guideline: documentation insufficient for assessment.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Deviations:

yes

Remarks:

8h exposure (instead of 7h); temperature 26°C (instead of 19-25°C); air flow 360 L/h (instead of 600L/h); further details on the method are lacking.

GLP compliance:

no

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2,2'-dimorpholinyldiethyl ether

EC Number:

229-194-7

EC Name:

2,2'-dimorpholinyldiethyl ether

Cas Number:

6425-39-4

Molecular formula:

C12H24N2O3

IUPAC Name:

4-{2-[2-(morpholin-4-yl)ethoxy]ethyl}morpholine

Details on test material:

- Name of test material (as cited in study report): 4236-14-25

Specific details on test material used for the study:

OTHER SPECIFICS:
- Name of test material (as cited in study report): 4236-14-25

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

Chamber conditions:
size: 80 liters
atmospheric pressure: 29.49 inches Hg
temperature: 26 °C
air flow: 6.0 l/min

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

clean air

Details on inhalation exposure:

Generation of test material:
The vapor was generated by bubbling a stream of filtered, dry air (-40°C dewpoint) through the undiluted test material in a gas washing bottle. The resulting air-vapor mixture was then introduced into the exposure chamber.

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

8 h

Concentrations:

nominal concentration: 0.21 mg/L air

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 0.21 mg/L air (nominal)

Based on:

test mat.

Exp. duration:

8 h

Mortality:

All animals survived

Clinical signs:

other: There were no untoward reactions during exposure or the 14-day observation period which followed.

Body weight:

The average 2-week body weight gain for all the test animals was within the normal limits.
weight change males: 74 grams
weigth change females: 28 grams

Gross pathology:

animal 10 (female): kidney: right hydronephrotic
animal 4 (male): lung: few minute red foci in all lobes
No gross tissue changes attributable to the effects of the test material were observed in any of the treated rats examined.

Applicant's summary and conclusion

Interpretation of results:

Category 1 based on GHS criteria

Conclusions:

The acute inhalation LC50 of the substance in rats was > 0.21 mg/L