Reaction mass of 3a,4,5,6,7,7a-hexahydro-4,7-methanoinden-5-yl acetate and 3a,4,5,6,7,7a-hexahydro-4,7-methanoinden-6-yl acetate

Toxicity to soil macroorganisms except arthropods

Description of key information

Cyclacet's earthworm EC10 is 57 mg/kg soil dw based on read across from Verdox, which was tested in an OECD TG 222. The values were converted for molecular weight and log Kow

Key value for chemical safety assessment

Long-term EC10, LC10 or NOEC for soil macroorganisms:

57 mg/kg soil dw

Additional information

The read across to Cyclacet is presented in the Terrestrial toxicity Overall Endpoint summary. The executive summary of the Verdox long-earthworm study is presented below.

The executive summary of the Verdox long-term earthworm study

The long-term toxicity of Verdox to earthworm Eisenia foetida was tested in an OECD TG 222 study. The test material was dissolved in equal amounts of acetone, mixed with quartz sand and allowed to slowly evaporate and mixed with the standard soil containing 10% Sphagnum peat, 20% kaolinite clay, approximately 70% fine quartz-sand (grain size 0.05 -0.2 mm) and 0.4% calcium carbonate to adjust to pH 6.0±0.5. Adult worms were exposed to nominal concentrations in soil of 5, 15.8, 50, 158.1 and 500 mg/kg dw soil. Weights of the adult worms ranged between 322 and 596 mg. The worms were fed weekly with finely ground cattle manure. Adult worms were removed after 4 weeks of exposure, counted and weighed. The remaining offspring remained in the test containers for another four weeks. Nominal concentrations are used to present the effects. No worms survived at 500 mg/kg soil dw. The NOEC for mortality and growth was 158.1 mg/kg dw. The EC10 for reproduction was 45.1 mg/kg dw soil (95 % confidence interval is 21-65 mg/kg soil dw).