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Ecotoxicological information

Long-term toxicity to fish

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Description of key information

The long-term fish toxicity of Cyclacet is 2.1 mg/l. This value is derived using the acute / chronic ratio of Cyclaprop, which is used for read across.

Key value for chemical safety assessment

EC10, LC10 or NOEC for freshwater fish:
2.1 mg/L

Additional information

The executive summary of the long-term fish toxicity of Cyclaprop is presented below. The documentation on the application of acute / chronic ratio and the read across to Cyclacet is presented in the overall aquatic endpoint summary.

Cyclaprop and its long-term fish toxicity

An early life-stage toxicity test was performed with the fathead minnow (Pimephales promelas) according to OECD TG 210 (v. 2013) and in compliance with GLP standards. Fish eggs aged less than 24 h were exposed in a flow-through system for 33 days (5 days to hatching and 28 days post hatch). Dimethyl formamide was used to prepare the stock solutions. A solvent control was included. Nominal test concentrations were 0.31, 0.63, 1.3, 2.5 and 5.0 mg/L whereas the mean measured concentrations were 0.20, 0.38, 0.80, 1.5 and 3.4 mg/L, respectively. These levels represented between 60 and 68% of the nominal concentrations. There were no significant differences between the effects in the control and the solvent control and so the data were pooled. The results are based on the mean measured concentrations. No significant treatment-related effects on survival were noted at concentrations up to and including 3.4 mg/L, whereas survival was affected starting at 1.5 mg/L. Sublethal effects (behaviour and morphology) were seen starting at a concentration of 1.5 mg/L and above. Growth (measured as total length, wet and dry weight), larval survival and sublethal effects were the determining endpoint with 33d-NOEC = 0.8 mg/L. The LOEC was 1.5 mg/L and the MATC was 1.1 mg/L. The lowest 33d-EC10 value was 0.86 (0.049 - 1.1) mg/L for survival; the 33d-EC20 value was 1.2 (0.97 - 1.3) mg/L.