Decyl oleate

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

disregarded due to major methodological deficiencies

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: GLP guideline study but not sufficient for this endpoint.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38412, part 27 (Pseudomonas oxygen consumtion inhibition test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Fatty acids, C16-18, C12-18-alkyl esters

EC Number:

306-083-2

EC Name:

Fatty acids, C16-18, C12-18-alkyl esters

Cas Number:

95912-87-1

IUPAC Name:

95912-87-1

Details on test material:

- Name of test material (as cited in study report): Fatty acids C16-18, C12-18-alkylesters, Cetylpalmitat herbal
- Physical state: solid
- Analytical purity: approx. 100% active substance
- Lot/batch No.: 10017345
- Expiration date of the lot/batch: 12-1999
- Stability under test conditions: not volatile at ambient temperature
- Other: hardly soluble, colour white

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance was weighted in directly to the dilution water and was treated with ultrasound. 2 mL glucose solution and 1 -3 mL
bacterial suspension were added.

Test organisms

Test organisms (species):

Pseudomonas putida

Details on inoculum:

- Laboratory culture: Pseudomonas putida MIGULA, strain Berlin 33/2 (DSM 50026)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

30 min

Test conditions

Test temperature:

20.8 - 21.1 °C

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 100 mL
- Aeration: directly

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to DIN 38412, part 27, point 8.1

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

30 min

Dose descriptor:

EC0

Effect conc.:

10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

30 min

Dose descriptor:

EC10

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

- Effect concentrations exceeding solubility of substance in test medium: yes, test substance is hardly soluble

Any other information on results incl. tables

The test substance turned out to be not toxic to the test organism Pseudomonas putida.