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EC number: 202-980-7 | CAS number: 101-83-7
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: other: preincubation method according to Ames, Mutat. Res. 31,347(1975); Maron, Mutat. Res.113,173(1983)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
Data source
Reference
- Reference Type:
- publication
- Title:
- Mutagenicity Test Data of Existing Chemical Substances
- Author:
- JETOC (Jap. Chem. Ind. Ecolog. Toxicol. Inform. Center)
- Year:
- 2 000
- Bibliographic source:
- JETOC (Jap. Chem. Ind. Ecolog. Toxicol. Inform. Center)Suppl. 2, 33-39, 71, 170-171.
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: according to Ames, Mutat. Res. 31,347(1975); Maron, Mutat. Res.113,173(1983)
- Principles of method if other than guideline:
- Preparation of S9 Fraction:
Male Sprague-Dawley rats were used for the preparation of liver fractions. Sodium phenobarbital and 5,6-benzofravone were used as an inducer of the rat metabolic activation system. Sodium phenobarbital was injected intraperitoneally into the rats 4 days before killing and 1, 2 and 3 days before killing 5,6-benzoflavone was injected intraperitoneally; from these rats liver S9 fraction was prepared according to Ames et al. (1975), Methods for detecting carcinogens and mutagens in the Salmonella /mammalian microsome mutagenicity test, Mutat. Res. 31, 347-364. S9 was dispensed into freezing ampules and stored at -80°C. Once the stock S9 had been thawed, remained S9 was not reused.
Evaluation criteria:
Twohold rule was used for data evaluation. the chemicals are considered to be mutagenic when dose-related increase in revertant colony count is observed and the number of revertant colonies per plate with the test substance is more than twice that of the negative control (solvent control) and when a reproducibility of test result is observed. - GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Dicyclohexylamine
- EC Number:
- 202-980-7
- EC Name:
- Dicyclohexylamine
- Cas Number:
- 101-83-7
- Molecular formula:
- C12H23N
- IUPAC Name:
- N-cyclohexylcyclohexanamine
- Details on test material:
- purity >99%
Constituent 1
Method
- Target gene:
- Salmonella typhimurium TA98, TA100, TA1535, TA1537, Escherichia coli WP2uvrA/pKM101
- Test concentrations with justification for top dose:
- (1) 0, 1.22, 4.88, 19.5, 78.1, 313, 1250, 5000 µg/plate in DMSO
(2) 0, 78.1, 156, 313, 625, 1250, 2500, 5000 µg/plate in DMSO - Vehicle / solvent:
- DMSO
Results and discussion
Test resultsopen allclose all
- Species / strain:
- E. coli WP2 uvr A pKM 101
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
negative
Applicant's summary and conclusion
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