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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
year of publication: 1992
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Sufficently documented literature data

Data source

Reference
Reference Type:
publication
Title:
Salmonella Mutagenicity Tests: V. Results from the testing of 311 chemicals
Author:
Zeiger E, Anderson B, Haworth S, Lawlor T, Mortelmans K
Year:
1992
Bibliographic source:
Env. Molecular Mutagenisis 19:2-141

Materials and methods

Principles of method if other than guideline:
Bacterial reverse mutation assay (Ames test), preincubation assay
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
2-chlorobenzaldehyde
EC Number:
201-956-3
EC Name:
2-chlorobenzaldehyde
Cas Number:
89-98-5
Molecular formula:
C7H5ClO
IUPAC Name:
2-chlorobenzaldehyde
Details on test material:
- Name of test material (as cited in study report): 2-chlorobenzaldehyde
- Analytical purity: 95.1%
- Impurities (identity and concentrations): no data

Method

Species / strain
Species / strain / cell type:
S. typhimurium, other: TA 97, TA 98, TA 100, TA 1535, TA 1537
Additional strain / cell type characteristics:
not specified
Metabolic activation:
with and without
Metabolic activation system:
S9-mix
Test concentrations with justification for top dose:
0 (control), 3.3, 10.0, 33.0, 100.0, 200.0, 333.0, 500.0, 666.0 µg/plate
Vehicle / solvent:
DMSO
Controls
Untreated negative controls:
no
Negative solvent / vehicle controls:
yes
Remarks:
DMSO
True negative controls:
no
Positive controls:
yes
Positive control substance:
other: without metabolic activation: sodium azide (TA1535 and TA100), 9-aminoacridine (TA97 and TA1537), 4-nitro-o-phenylenediamine (TA 98 and 1538); with metabolic activation: 2-aminoanthracene (all strains)
Details on test system and experimental conditions:
20 min preincubation procedure

Results and discussion

Test results
Species / strain:
S. typhimurium, other: TA 97, TA 98, TA 100, TA 1535, TA 1537
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Additional information on results:
It was stated in the text, that maximum concentrations of 10000 µg/plate are only tested if substances were not toxic. As 2-chlorobenzaldehyde was only tested up to 666 µg/plate it can be concluded, that it was toxic at higher concentrations. But, no results of the testing for cytoxicity were presented in the publication.
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation

2-Chlorobenzaldehyde was not mutagenic under the conditions tested.
Executive summary:

2-Chlorobenzaldehyde was not mutagenic in the bacterial reverse mutation assay (Ames test, test strains: Salmonella typhimurium TA 97, TA 98, TA 100, TA 1535, TA 1537) with and without metabolic activation (S9 -mix).

This well documented and reliable (RL2) study has been selected as key study for the evaluation of gene mutations.