2-chlorobenzaldehyde

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

year of publication: 1992

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Sufficently documented literature data

Data source

Reference

Reference Type:

publication

Title:

Salmonella Mutagenicity Tests: V. Results from the testing of 311 chemicals

Author:

Zeiger E, Anderson B, Haworth S, Lawlor T, Mortelmans K

Year:

1992

Bibliographic source:

Env. Molecular Mutagenisis 19:2-141

Materials and methods

Principles of method if other than guideline:

Bacterial reverse mutation assay (Ames test), preincubation assay

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

2-chlorobenzaldehyde

EC Number:

201-956-3

EC Name:

2-chlorobenzaldehyde

Cas Number:

89-98-5

Molecular formula:

C7H5ClO

IUPAC Name:

2-chlorobenzaldehyde

Details on test material:

- Name of test material (as cited in study report): 2-chlorobenzaldehyde
- Analytical purity: 95.1%
- Impurities (identity and concentrations): no data

Method

Species / strain

Species / strain / cell type:

S. typhimurium, other: TA 97, TA 98, TA 100, TA 1535, TA 1537

Additional strain / cell type characteristics:

not specified

Metabolic activation:

with and without

Metabolic activation system:

S9-mix

Test concentrations with justification for top dose:

0 (control), 3.3, 10.0, 33.0, 100.0, 200.0, 333.0, 500.0, 666.0 µg/plate

Vehicle / solvent:

DMSO

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

Remarks:

DMSO

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: without metabolic activation: sodium azide (TA1535 and TA100), 9-aminoacridine (TA97 and TA1537), 4-nitro-o-phenylenediamine (TA 98 and 1538); with metabolic activation: 2-aminoanthracene (all strains)

Details on test system and experimental conditions:

20 min preincubation procedure

Results and discussion

Test results

Species / strain:

S. typhimurium, other: TA 97, TA 98, TA 100, TA 1535, TA 1537

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Untreated negative controls validity:

not specified

Positive controls validity:

not specified

Additional information on results:

It was stated in the text, that maximum concentrations of 10000 µg/plate are only tested if substances were not toxic. As 2-chlorobenzaldehyde was only tested up to 666 µg/plate it can be concluded, that it was toxic at higher concentrations. But, no results of the testing for cytoxicity were presented in the publication.

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation

2-Chlorobenzaldehyde was not mutagenic under the conditions tested.

Executive summary:

2-Chlorobenzaldehyde was not mutagenic in the bacterial reverse mutation assay (Ames test, test strains: Salmonella typhimurium TA 97, TA 98, TA 100, TA 1535, TA 1537) with and without metabolic activation (S9 -mix).

This well documented and reliable (RL2) study has been selected as key study for the evaluation of gene mutations.