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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From August 14th to September 5th, 2000
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report Date:
2000

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
1992
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
Due to the very low water solubility of the test item, a supersaturated stock dispersion of the test item with a loading rate of nominal 100 mg/I was continuously stirred at room temperature in the dark over 96 hours. Then the dispersion was filtered. The undiluted filtrate with the maximum concentration of dissolved test item was used as the test medium.
Additionally, a control was tested in parallel.

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
7 fish in control
7 fish in test item concentration

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h

Test conditions

Hardness:
250 mg CaCO3/l
Test temperature:
23 °C
pH:
7.8-8
Dissolved oxygen:
>= 8 mg/l
Nominal and measured concentrations:
Nominal 100 mg/l as loading rate
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LOEC
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
The 96-hour NOEC (highest concentration tested without toxic effects after the exposure period of 96 hours), and the 96-hour LC0 of test item to zebra fish were determined to be at least at a loading rate of 100 mg/I. The 96-hour NOEC and the 96-hour LC0 might even be higher than this loading rate, but loading rates above 100 mg/I were not tested, according to the guidelines.
The 96-hour LOEC (lowest concentration with toxic effects) and the 96-hour LC50 were clearly higher than the loading rate of 100 mg/I. These values could not be quantified, since the test item had no toxic effect up to the highest concentration which could be dissolved in the test water.

The biological results are related to the loading rates of 100 mg/l. No effects were observed at the solubility limit of < 0.8 µg/l (detection limit). In conclusion, the LC50 is > 0.8 µg/l, the NOEC 0.8 µg/l.

Applicant's summary and conclusion

Conclusions:
Test item has no toxic effect on the fish up to its solubility limit in test water.