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EC number: 202-684-8 | CAS number: 98-59-9
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1978
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- The study was performed pre-GLP and according to a method similar to OECD471 but with some deviations: - Single plates were used instead of triplicate plates. - With this test it is not possible to identify certain oxidising mutagens, cross-linking agents and hydrazines. Such substances may be detected by E.coli WP2 strains or S. typhimurium TA102 which have an AT base pair at the primary reversion site in stead of GC base pairs which the strains tested in this study have. - 3 concentration tested at which no cytotoxicity was observed instead of 5.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 978
- Report date:
- 1978
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Principles of method if other than guideline:
- - Single plates were used instead of triplicate plates.
- With this test it is not possible to identify certain oxidising mutagens, cross-linking agents and hydrazines. Such substances may be detected by E.coli WP2 strains or S. typhimurium TA102 which have an AT base pair at the primary reversion site in stead of GC base pairs which the strains tested in this study have.
- 3 concentration tested at which no cytotoxicity was observed instead of 5 - GLP compliance:
- no
- Remarks:
- pre-GLP
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Tosyl chloride
- EC Number:
- 202-684-8
- EC Name:
- Tosyl chloride
- Cas Number:
- 98-59-9
- Molecular formula:
- C7H7ClO2S
- IUPAC Name:
- 4-methylbenzenesulfonyl chloride
- Details on test material:
- Identification: Para-Toluene Sulpho Chloride
Date Received: March 27, 1978
Physical Description: White crystals
No further data
Constituent 1
Method
- Target gene:
- Histidine operon
Species / strainopen allclose all
- Species / strain / cell type:
- other: S. typhimurium TA 1535, TA 1537, TA 98, TA 1538 and TA 100
- Species / strain / cell type:
- Saccharomyces cerevisiae
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-mix
- Test concentrations with justification for top dose:
- 1.0, 10.0, 100.0, 500.0 and 1000.0 microgram/plate
Controls
- Untreated negative controls:
- yes
- Remarks:
- DMSO solvent
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO solvent
- True negative controls:
- no
- Positive controls:
- yes
- Remarks:
- depending to strain
- Positive control substance:
- 2-nitrofluorene
- ethylmethanesulphonate
- other: Quinacrine mustard; Anthraceen
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Species / strain:
- Saccharomyces cerevisiae
- Remarks:
- D4
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- (except at 500 and 1000µg/plate)
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Remarks:
- There is no know positive compound that works wwith this strain in the activation plate assay.
Any other information on results incl. tables
The test compound exhibited toxicity with all the strains except D4 at 500 and 1000 ug per plate dose level. The results of the tests conducted on the compound in the presence and absence of a metabolic activation system were all negative.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
other: study is invalid no reliable conclusion can be drawn
Tosyl chloride was not mutagenic in the Ames Salmonella/microsome plate test with and without metabolic activation. - Executive summary:
The study was performed pre-GLP and according to a method similar to OECD 471 (plate incorporation). S. typhimurium TA 1535, TA 1537, TA 98, TA 1538 and TA 100 and Saccharomyces cerevisiae were exposed to 1.0, 10.0, 100.0, 500.0 and 1000.0 microgram/plate. The negative control was the solvent DMSO. The postitive control depended on the strain.
The study was performed with some deviations compared to OECD 471:
- Single plates were used instead of triplicate plates.
- With this test it is not possible to identify certain oxidising mutagens, cross-linking agents and hydrazines. Such substances may be detected by E.coli WP2 strains or S. typhimurium TA102 which have an AT base pair at the primary reversion site in stead of GC base pairs which the strains tested in this study have.
- 3 concentration tested at which no cytotoxicity was observed instead of 5.
The test compound exhibited toxicity with all the strains except D4 at 500 and 1000 ug per plate dose level. The results of the tests conducted on the compound in the presence and absence of a metabolic activation system were all negative.The authors concluded that the outcome of the study is negative.
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