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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 402 (Acute Dermal Toxicity)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
But-2-yne-1,4-diol
EC Number:
203-788-6
EC Name:
But-2-yne-1,4-diol
Cas Number:
110-65-6
Molecular formula:
C4H6O2
IUPAC Name:
but-2-yne-1,4-diol
Details on test material:
- Name of test material (as cited in study report): Butindiol
- Analytical purity: 98.9 % (2.TV), 98.9 % (1.TV)

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Hoechst AG
- Age at study initiation: male: 7 - 8 weeks, female: 8 -9 weeks
- Weight at study initiation: male: 209 g +/- 12 g female: 199 g +/- 10 g
- Housing: individually
- Diet: ad libitum
- Water: ad libitum
- Acclimation period: 5 days


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 3 °C
- Humidity (%): 50 +/- 20 %
- Photoperiod (hrs dark / hrs light): 12 hours

Administration / exposure

Type of coverage:
occlusive
Vehicle:
physiological saline
Details on dermal exposure:
TEST SITE
- Area of exposure: 30 cm²

REMOVAL OF TEST SUBSTANCE
- Washing (if done): after 24 hours, warm water
Duration of exposure:
24 hours
Doses:
male: 50, 100, 200, 400, 2000 mg/kg b.w.
female: 400, 1250, 1600, 2000 mg/kg b.w.
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days

Results and discussion

Effect levelsopen allclose all
Sex:
male/female
Dose descriptor:
LD50
Effect level:
659 mg/kg bw
Based on:
test mat.
95% CL:
324 - 1 440
Sex:
male
Dose descriptor:
other: NOAEL
Effect level:
50 mg/kg bw
Based on:
test mat.
Clinical signs:
other: reduced spontaneous activity, crouch, retacted flank, constricted palpebral fissure, piloerection, decreased respiratory frequency, high-stepping gait, unsteady gait

Any other information on results incl. tables

Mortality:

 Dose (mg/kg b.w.)  Male animals  Female animals
 50  0/5  -
 100  1/5  -
 200  2/5  -
 400  2/5  0/5
 1250  3/5
 1600  -  3/5
 2000  4/5  5/5

LD50 male animals: 424 (178 - 1880) mg/kg b.w.;          
LD50 female animals: 983 (307 - 2440) mg/kg b.w.;
LD50 male + female animals: 659 (324 - 1440) mg/kg b.w.

Applicant's summary and conclusion

Interpretation of results:
toxic
Remarks:
Migrated information Category 3 Criteria used for interpretation of results: EU