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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Meets national standard methods (DIN).

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report date:
1988

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: German standard DIN 38412, part 8
Principles of method if other than guideline:
Growth-inhibition test according to Bringmann-Kühn.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
3-methylbut-3-en-1-ol
EC Number:
212-110-8
EC Name:
3-methylbut-3-en-1-ol
Cas Number:
763-32-6
Molecular formula:
C5H10O
IUPAC Name:
3-methylbut-3-en-1-ol
Details on test material:
- Name of test material (as cited in study report): 3-Methylbuten-3-ol-1
no further details on the test substance characteristics given

Sampling and analysis

Analytical monitoring:
no

Test organisms

Test organisms (species):
Pseudomonas putida
Details on inoculum:
Pseudomonas putida, strain DSM 50026

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
17 h

Test conditions

Test temperature:
24 +/- 1 °C
Nominal and measured concentrations:
Nominal Test concentrations (mg/l): 312.5, 625, 1250, 2500, 5000, 7500, 10000, control
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer flasks (nominal volume 300 ml)


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: AK medium (DIN 38412)

Results and discussion

Effect concentrationsopen allclose all
Duration:
17 h
Dose descriptor:
EC10
Effect conc.:
1 350 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Duration:
17 h
Dose descriptor:
EC50
Effect conc.:
1 870 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Duration:
17 h
Dose descriptor:
other: EC90
Effect conc.:
2 400 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition

Any other information on results incl. tables

EC10 = 1349.6 mg/l
EC50 = 1874.2 mg/l
EC90 = 2398.8 mg/l

pH values (range over all concentrations):
test preparation, uninoculated, at 0 h: 6.7 - 7.2; blank: 7.1
test preparation, uninoculated, at 17 h: 6.7 - 7.1; blank: 7.1
parallel no. 1, at 17 h: 5.6 - 6.7; blank: 5.6

Applicant's summary and conclusion