Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Currently viewing:

Administrative data

Link to relevant study record(s)

Referenceopen allclose all

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
June to July 1994
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: This robust summary has a reliability rating of 1 because the study followed a standard guideline, followed GLP guidelines, and was conducted without deviations that would invalidate the study.
Justification for type of information:
A discussion and report on the read across strategy is given as an attachment in IUCLID Section 13.
Reason / purpose:
read-across: supporting information
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
The water accommodated fraction (WAF) of the test material was prepared by stirring the test material in the exposure solution for approximately 24 hours. The stirring was as vigorous as possible without causing an emulsion to form. After stirring, the WAF was allowed to settle for 1 hour before removing the aqueous phase for testing.
Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
Organisms used in the test were from a laboratory culture, originally derived from a strain (ATCC 22662) obtained from the American Type Culture Collection, Maryland, Ohio, USA.
Test type:
static
Limit test:
yes
Total exposure duration:
72 h
Post exposure observation period:
None
Test temperature:
24 to 26 degrees C
pH:
Within Guideline requirements
Nominal and measured concentrations:
The nominal loading rate was 1000 mg/L. A control was also tested.
Details on test conditions:
The study was conducted in sealed test systems with no headspace that were not renewed during the study. The test systems used were 300 ml glass Erlenmeyer flasks. The treatment level was evaluated in triplicate test systems and the control in 6 replicate test systems. Control and treatment systems were innoculated with a concentration of 5000 cells/ml. Test systems were incubated under constant illumination (approximately 3000 lux) on an orbital incubator.
Reference substance (positive control):
no
Key result
Duration:
72 h
Dose descriptor:
EL50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
cell number
Key result
Duration:
72 h
Dose descriptor:
EL50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
NOELR
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
cell number
Key result
Duration:
72 h
Dose descriptor:
NOELR
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Reported statistics and error estimates:
The growth of algae in the the test systems was greater than the controls, which performed normally, therefore no analyses were required.
Validity criteria fulfilled:
yes
Conclusions:
Growth of alga cultures, as measured by biomass and growth rate, exposed to a water accommodated fraction of the test substance was not inhibited over 72 hours. The growth of algae in the the test systems was greater than the controls, which performed normally. Therefore, the 72-hr EL50 values for the two endpoints are reported as >1000 mg/L. The 72-hr NOELR values for biomass and growth rate are reported as 1000 mg/L, respectively.
Executive summary:

Growth of alga cultures, as measured by biomass and growth rate, exposed to a water accommodated fraction of the test substance was not inhibited over 72 hours. The growth of algae in the the test systems was greater than the controls, which performed normally. Therefore, the 72-hr EL50 values for the two endpoints are reported as >1000 mg/L. The 72-hr NOELR values for biomass and growth rate are reported as 1000 mg/L, respectively.

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
June 1994
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: This robust summary has a reliability rating of 1 because the study followed a standard guideline, followed GLP guidelines, and was conducted without deviations that would invalidate the study.
Justification for type of information:
A discussion and report on the read across strategy is given as an attachment in IUCLID Section 13.
Reason / purpose:
read-across: supporting information
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
The water accommodated fraction of the test material were prepared by stirring the test material in the exposure solution for 25 hours after which the the aqueous phase was removed for testing.
Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
Organisms used in the test were from a laboratory culture, originally derived from a strain (ATCC 22662) obtained from the American Type Culture Collection, Maryland, Ohio, USA.
Test type:
static
Limit test:
yes
Total exposure duration:
72 h
Post exposure observation period:
None
Test temperature:
24 to 26 degrees C
pH:
Within Guideline requirements
Nominal and measured concentrations:
The nominal loading rate was 1000 mg/L. A control was also tested.
Details on test conditions:
The study was conducted in sealed test systems with no headspace that were not renewed during the study. The test systems used were 300 ml glass Erlenmeyer flasks. The treatment level was evaluated in triplicate test systems and the control in 6 replicate test systems. Control and treatment systems were innoculated with a concentration of 5000 cells/ml. Test systems were incubated under constant illumination (approximately 3000 lux) on an orbital incubator.
Reference substance (positive control):
no
Key result
Duration:
72 h
Dose descriptor:
EL50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
cell number
Key result
Duration:
72 h
Dose descriptor:
EL50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
NOELR
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
cell number
Key result
Duration:
72 h
Dose descriptor:
NOELR
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Reported statistics and error estimates:
The growth of algae in the the test systems was greater than the controls, which performed normally, therefore no analyses were required.
Validity criteria fulfilled:
yes
Conclusions:
Growth of alga cultures, as measured by biomass and growth rate, exposed to a water accommodated fraction of the test substance was not inhibited over 72 hours. The growth of algae in the the test systems was greater than the controls, which performed normally. Therefore, the 72-hr EL50 values for the two endpoints are reported as >1000 mg/L. The 72-hr NOELR values for biomass and growth rate are reported as 1000 mg/L, respectively.
Executive summary:

Growth of alga cultures, as measured by biomass and growth rate, exposed to a water accommodated fraction of the test substance was not inhibited over 72 hours. The growth of algae in the the test systems was greater than the controls, which performed normally. Therefore, the 72-hr EL50 values for the two endpoints are reported as >1000 mg/L. The 72-hr NOELR values for biomass and growth rate are reported as 1000 mg/L, respectively.

Description of key information

Growth of alga cultures, as measured by biomass and growth rate, exposed to a water accommodated fraction of hydrocarbons, C10-C12, isoalkanes, <2% aromatics, was not inhibited over 72 hours. The growth of algae in the the test systems was greater than the controls, which performed normally. Therefore, the 72-hr EL50 values for the two endpoints are reported as >1000 mg/L. The 72-hr NOELR values for biomass and growth rate are reported as 1000 mg/L, respectively.

Growth of alga cultures, as measured by biomass and growth rate, exposed to a water accommodated fraction of hydrocarbons, C10-C13, n-alkanes, isoalkanes, cyclics, <2% aromatics,  was not inhibited over 72 hours. The growth of algae in the the test systems was greater than the controls, which performed normally. Therefore, the 72-hr EL50 values for the two endpoints are reported as >1000 mg/L. The 72-hr NOELR values for biomass and growth rate are reported as 1000 mg/L, respectively.

The data from these two studies are used as read-across data to hydrocarbons, C10 -C13, isoalkanes, cyclics, <2% aromatics.

Key value for chemical safety assessment

Additional information

Growth of alga cultures, as measured by biomass and growth rate, exposed to a water accommodated fraction of hydrocarbons, C10-C12, isoalkanes, <2% aromatics, was not inhibited over 72 hours. The growth of algae in the the test systems was greater than the controls, which performed normally. Therefore, the 72-hr EL50 values for the two endpoints are reported as >1000 mg/L. The 72-hr NOELR values for biomass and growth rate are reported as 1000 mg/L, respectively.

Growth of alga cultures, as measured by biomass and growth rate, exposed to a water accommodated fraction of hydrocarbons, C10-C13, n-alkanes, isoalkanes, cyclics, <2% aromatics, was not inhibited over 72 hours. The growth of algae in the the test systems was greater than the controls, which performed normally. Therefore, the 72-hr EL50 values for the two endpoints are reported as >1000 mg/L. The 72-hr NOELR values for biomass and growth rate are reported as 1000 mg/L, respectively.

The data from these two studies are used as read-across data to hydrocarbons, C10 -C13, isoalkanes, cyclics, <2% aromatics.