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Short-term toxicity to aquatic invertebrates

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Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
May 1994
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: This robust summary has a reliability rating of 1 because the study followed a standard guideline, followed GLP guidelines, and was conducted without deviations that would invalidate the study.
Justification for type of information:
A discussion and report on the read across strategy is given as an attachment in IUCLID Section 13.
Reason / purpose:
read-across: supporting information
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
The water accommodated fraction (WAF) of the test material was prepared by stirring the test material in the exposure solution for approximately 24 hours. The stirring was as vigorous as possible without causing an emulsion to form. After stirring, the WAF was allowed to settle for 1 hour before removing the aqueous phase for testing.
Test organisms (species):
Daphnia magna
Details on test organisms:
Organisms used in the test were from a laboratory culture were less than 24 hour old. The source of the culture was from Zeneca Brixham Laboratory from I.R.Ch.A., France.
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
None
Hardness:
196 mg/L CaCO3
Test temperature:
18 to 20 degrees C
pH:
Within Guideline requirement
Dissolved oxygen:
8.9 to 9.2 mg/L
Nominal and measured concentrations:
The nominal loading rate was 1000 mg/L. A control was also tested.
Details on test conditions:
The study was conducted in closed test systems that were not renewed during the study. The test systems used were 150 ml glass Erlenmeyer flasks. The control and treatment level was evaluated in replicate test systems. Ten organisms, less than 24 hours old, were exposed in each of the control and treatment solution flasks and were not fed during the study.
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
EL50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
LL0
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
48 h
Dose descriptor:
EL50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EL0
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
There was no immobility reported in the treatment and control solutions at test termination.
Reported statistics and error estimates:
None
Validity criteria fulfilled:
yes
Conclusions:
The water accommodated fraction (WAF) of the test substance did not produce a 50% effect (immobility) with Daphnia magna at a loading of 1000 mg/L after a 48-hour exposure. Therefore, the 48-hour LL50 is reported as >1000 mg/L. There was no mortality at the 1000 mg/L loading level after 48 hours. Therefore, the 48-hour LL0 for mortality is reported as 1000 mg/L. There was also no mortality in the control.
Executive summary:

The water accommodated fraction (WAF) of the test substance did not produce a 50% effect (immobility) with Daphnia magna at a loading of 1000 mg/L after a 48-hour exposure. Therefore, the 48-hour LL50 is reported as >1000 mg/L. There was no mortality at the 1000 mg/L loading level after 48 hours. Therefore, the 48-hour LL0 for mortality is reported as 1000 mg/L. There was also no mortality in the control.

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
May 1994
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: This robust summary has a reliability rating of 1 because the study followed a standard guideline, followed GLP guidelines, and was conducted without deviations that would invalidate the study.
Justification for type of information:
A discussion and report on the read across strategy is given as an attachment in IUCLID Section 13.
Reason / purpose:
read-across: supporting information
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
The water accommodated fraction of the test material was prepared by stirring the test material in the exposure solution for 22 hours after which the the aqueous phase was removed for testing.
Test organisms (species):
Daphnia magna
Details on test organisms:
Organisms used in the test were from a laboratory culture were less than 24 hour old. The source of the culture was from Zeneca Brixham Laboratory from I.R.Ch.A., France.
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
None
Hardness:
196 mg/L CaCO3
Test temperature:
18 to 20 degrees C
pH:
Within Guideline requirement
Dissolved oxygen:
9.0 mg/L
Nominal and measured concentrations:
The nominal loading rate was 1000 mg/L. A control was also tested.
Details on test conditions:
The study was conducted in closed test systems that were not renewed during the study. The test systems used were 150 ml glass Erlenmeyer flasks. The control and treatment level was evaluated in replicate test systems. Ten organisms, less than 24 hours old, were exposed in each of the control and treatment solution flasks and were not fed during the study.
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
EL50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
LL0
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
48 h
Dose descriptor:
EL50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EL0
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
There was no immobility reported in the treatment and control solutions at test termination.
Reported statistics and error estimates:
None
Validity criteria fulfilled:
yes
Conclusions:
The water accommodated fraction (WAF) of the test substance did not produce a 50% effect (immobility) with Daphnia magna at a loading of 1000 mg/L after a 48-hour exposure. Therefore, the 48-hour LL50 is reported as >1000 mg/L. There was no mortality at the 1000 mg/L loading level after 48 hours. Therefore, the 48-hour LL0 for mortality is reported as 1000 mg/L. There was also no mortality in the control.
Executive summary:

The water accommodated fraction (WAF) of the test substance did not produce a 50% effect (immobility) with Daphnia magna at a loading of 1000 mg/L after a 48-hour exposure. Therefore, the 48-hour LL50 is reported as >1000 mg/L. There was no mortality at the 1000 mg/L loading level after 48 hours. Therefore, the 48-hour LL0 for mortality is reported as 1000 mg/L. There was also no mortality in the control.

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
August 1983
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: This robust summary has a reliability rating of 2 because the study did not follow a GLP guideline and included limited water quality results, but did appear to use a standard procedure and generally acceptable testing procedures.
Justification for type of information:
A discussion and report on the read across strategy is given as an attachment in IUCLID Section 13.
Reason / purpose:
read-across: supporting information
Qualifier:
according to
Guideline:
other: U.S. Environmental Protection Agency (1978) Bioassay procedures for the ocean disposal permit program EPA-600/9-78-010
Deviations:
no
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
The treatment solutions were prepared as percent dilutions of a 100% water soluble fraction (WSF). The 100% WSF was prepared by adding 1 part of test material to 9 parts of artificial seawater (v:v). The mixture was stirred for 20 hours. After mixing, the aqueous phase was removed for further dilution to prepare the treatment levels, which were 10, 25, 40, 60, and 100% of the water soluble fraction.
Test organisms (species):
Americamysis bahia (previous name: Mysidopsis bahia)
Details on test organisms:
Test organisms were obtained from an in-house culture, cultivated under test conditions.
Test type:
static
Water media type:
saltwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
None
Test temperature:
19 to 21 degrees C
pH:
7.8
Dissolved oxygen:
7.0 to 7.4 mg/L
Nominal and measured concentrations:
The nominal treatment levels were 10, 25, 40, 60, and 100% of the water soluble fraction.
Details on test conditions:
The test material was evaluated in three separate tests. Each test exposed 30 organisms to a range of water soluble fractions (WSFs) of the test substance including a 100% WSF.
Reference substance (positive control):
no
Key result
Duration:
96 h
Dose descriptor:
LL50
Effect conc.:
> 81 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
Although mortality was observed in some of the treatment levels, which were dilutions of the 100% water soluble fraction (WSF) of the test substance prepared at a loading of 81,000 mg/L, a clear dose dependent relationship was not observed and mortality did not exceed 20%. The dilutions are represented as percents of the 100% WSF. A control was also tested.

Immobility results from the second definitive test:
Exposure Total
Solution Mortality*
(% WSF) (Test 1, 2, 3 @ 96 hrs)
Control 0, 0, 0
10 0, 0, 0
25 1, 0, 1
40 0, 0, 0
60 0, 0, 1
100 0, 2, 1

* 30 organisms tested in each of the control and treatment levels
Reported statistics and error estimates:
Statistical analyses were not conducted.
Validity criteria fulfilled:
yes
Conclusions:
The 100% water soluble fraction (WSF) of the test substance at a loading of 81,000 mg/L did not produce a 50% mortality with Mysidopsis bahia after a 96-hour exposure. Therefore, the 496-hour LL50 value is reported as >81,000 mg/L. There was also no mortality in the control.
Executive summary:

The 100% water soluble fraction (WSF) of the test substance at a loading of 81,000 mg/L did not produce a 50% mortality with Mysidopsis bahia after a 96-hour exposure. Therefore, the 496-hour LL50 value is reported as >81,000 mg/L. There was also no mortality in the control.

Description of key information

Freshwater:

Water accommodated fractions of hydrocarbons, C10-C12, isoalkanes, <2% aromatics, did not produce a 50% effect (immobility) with Daphnia magna at a loading of 1000 mg/L after a 48-hour exposure. Therefore, the 48-hour LL50 is reported as >1000 mg/L. There was no mortality at the 1000 mg/L loading level after 48 hours. Therefore, the 48-hour LL0 for mortality is reported as 1000 mg/L. There was also no mortality in the control.

The water accommodated fraction (WAF) of hydrocarbons, C10-C13, n-alkanes, isoalkanes, cyclics, <2% aromatics, did not produce a 50% effect (immobility) with Daphnia magna at a loading of 1000 mg/L after a 48-hour exposure. Therefore, the 48-hour LL50 is reported as >1000 mg/L. There was no mortality at the 1000 mg/L loading level after 48 hours. Therefore, the 48-hour LL0 for mortality is reported as 1000 mg/L. There was also no mortality in the control.

The data from these two studies are used as read-across data to hydrocarbons, C10-C13, isoalkanes, cyclics, <2% aromatics.

Marine:

The 100% water soluble fraction (WSF) of hydrocarbons, C10-C14, isoalkanes, cyclics, <2% aromatics, at a loading of 81,000 mg/L did not produce a 50% mortality with Mysidopsis bahia after a 96-hour exposure. Therefore, the 96-hour LL50 value is reported as >81,000 mg/L. There was also no mortality in the control.

These data are used as read-across data to hydrocarbons, C10-C13, isoalkanes, cyclics, <2% aromatics.

Key value for chemical safety assessment

Additional information

Freshwater:

Water accommodated fractions of hydrocarbons, C10-C12, isoalkanes, <2% aromatics, did not produce a 50% effect (immobility) with Daphnia magna at a loading of 1000 mg/L after a 48-hour exposure. Therefore, the 48-hour LL50 is reported as >1000 mg/L. There was no mortality at the 1000 mg/L loading level after 48 hours. Therefore, the 48-hour LL0 for mortality is reported as 1000 mg/L. There was also no mortality in the control.

The water accommodated fraction (WAF) of hydrocarbons, C10-C13, n-alkanes, isoalkanes, cyclics, <2% aromatics, did not produce a 50% effect (immobility) with Daphnia magna at a loading of 1000 mg/L after a 48-hour exposure. Therefore, the 48-hour LL50 is reported as >1000 mg/L. There was no mortality at the 1000 mg/L loading level after 48 hours. Therefore, the 48-hour LL0 for mortality is reported as 1000 mg/L. There was also no mortality in the control.

The data from these two studies are used as read-across data to hydrocarbons, C10-C13, isoalkanes, cyclics, <2% aromatics.

Marine:

The 100% water soluble fraction (WSF) of hydrocarbons, C10-C14, isoalkanes, cyclics, <2% aromatics, at a loading of 81,000 mg/L did not produce a 50% mortality with Mysidopsis bahia after a 96-hour exposure. Therefore, the 96-hour LL50 value is reported as >81,000 mg/L. There was also no mortality in the control.

These data are used as read-across data to hydrocarbons, C10-C13, isoalkanes, cyclics, <2% aromatics.