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EC number: 202-829-5 | CAS number: 100-20-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- This study is used for read-across and therefore has been assigned a reliability of 2 (reliable with restrictions). The study, if used in support of terephthalic acid, has a reliability of 1 (reliable without restriction). Proprietary GLP guideline study.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Remarks:
- The study was conducted according to the guideline in effect, at the time the study was conducted.
- GLP compliance:
- yes
- Vehicle:
- no
- Details on test solutions:
- The test substance and the reference were tested at different concentrations in the test solution containing 16 mL of the synthetic sewage feed, 200 mL of the inoculum, and deionised water to give 500 mL final volume.
In the range finding test, special measures were introduced to achieve a correct concentration of the poorly water soluble test material within the test solution at the low concentrations. For this purpose 100.00 mg of the test material were weighed into a measuring flask and dissolved in ethylacetate. 50, 5, and 0.5 mL of this solution were transferred into the incubation flasks. The solvent was evaporated. By this procedure the test material was distributed in the incubation flask as a very thin layer. The test solutions were then prepared like the blank by adding 284 mL deionised water and 16 mL of the synthetic sewage. The test was then started by addition of 200 mL activated sludge. - Test organisms (species):
- activated sludge
- Details on inoculum:
- Activated sludge from the sewage plant at Frankfurt a.M./Niederrad dealing predominantly with domestic sewage was washed twice with tap water. Dry weight was determined to be 14.49 g/L in the range-finding test, and 8.88 g/L in the definitive test. The activated sludge was diluted with tap water resulting in 4.0 g Dry mass/L. To this sludge suspension 50 mL of synthetic sewage was added per litre of activated sludge and the mixture was aerated for two days at room temperature until use in the test as inoculum. Each day it was fed with synthetic sewage concentrate.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- 10 minutes
- Hardness:
- Not Reported
- Test temperature:
- Not Reported
- pH:
- Not Reported
- Dissolved oxygen:
- Not Reported
- Salinity:
- Not Reported
- Nominal and measured concentrations:
- Test Substance
Range-finding test: 0.001, 0.01 and 0.1 g/L
Definitive test: 0, 0.5, 1.00, 2.00 and 4.00 g/L
Reference Substance
Range-finding test: 7.50, 15.00, and 30.00 mg/L
Definitive test: 7.0, 14.0, 30.0, and 60.0 mg/L - Details on test conditions:
- 1 L glass bottles previously washed carefully with methanol/HCl (90/10) served as test vessels. Synthetic sewage feed was mixed with the appropriate amount of test substance (four different concentrations within the definitive test [500, 1000, 2000, and 4000 mg/L], three concentrations in the range-finding-test [1, 10, and 100 mg/L]) and made up to 300 mL with deionised water. The test was started by adding 200 mL of the prepared activated sludge (4 g dry weight/L) to give 1.6 g dry weight/L final concentration in 500 mL final volume. Each test solution was aerated with compressed air by means of glass pipettes for exactly 3 h. At the end of the 3 h exposure period the solutions were poured into an oxygen-bottle (ORI) and oxygen consumption was recorded for 10 minutes.
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol at test concentrations of 7.5, 15 and 30 mg/L
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1 393 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 95% confidence interval: 1272-1524 mg/L
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- In the range finding test there was no effect on respiration. In the definitive test, the highest concentration of 4000 mg/L resulted in 95.8% inhibition of respiration, 2000 mg/L resulted in 93.4% inhibition, 1000 mg/L resulted in 29.7% inhibition, whilst 500 mg/L did not inhibit respiration.
- Results with reference substance (positive control):
- In the range finding test, 85.8% inhibition was achieved at 30 mg/L. In the definitive test, 97% inhibition was achieved at 30 mg/L
- Reported statistics and error estimates:
- The percent inhibition was plotted against concentration (log), and an EC50 value was calculated according to Litchfield and Wilcoxon with 95% confidence limits.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Inhibition, relative to the mean control rate occurred in the test substance treatments ≥1000 mg/L and the 3-h EC50 was 1392.8 mg/L. The respiration of activated sludge was unaffected by 3-h exposure to terephthalic acid at nominal concentrations of up to 500 mg/L. Therefore, a NOEC of 500 mg/L has been established.
- Executive summary:
The toxicity of the test substance was determined in an activated sludge respiration inhibition test according to OECD guideline 209. Activated sludge obtained from a predominantly domestic sewage plant was exposed over a period of three hours to the test substance at nominal concentrations of 500, 1000, 2000 and 4000 mg/L. A pair of test vessels was allocated to the control treatment and the test substance treatments were run singly. Inhibition, relative to the mean control rate occurred in the test substance treatments ≥1000 mg/L and the 3-h EC50 was 1393 mg/L. The respiration of activated sludge was unaffected by 3-h exposure to terephthalic acid at nominal concentrations of up to 500 mg/L. Therefore, a NOEC of 500 mg/L has been established.
Reference
Duration |
Endpoint |
Effect conc. |
3 h |
EC50 |
1393 mg/L |
3 h |
NOEC |
500 mg/L |
3 h |
EC75 |
1903 mg/L |
3 h |
EC95 |
2981.7 mg/L |
The results of the definitive test are presented in the table below:
Terephthalic acid nominal concentration (mg/L) |
respiration rate, mg O2/L*h |
respiration inhibition (%) relative to mean control |
0.0 |
153.6 |
n.a. |
500 |
180 |
0* |
1000 |
108 |
29.7 |
2000 |
10.2 |
93.4 |
4000 |
6.5 |
95.8 |
7.5 mg 3,5-DCP/L (reference inhibitor) |
51.6 |
66.4 |
15.0 mg 3,5-DCP-L (reference inhibitor) |
10.2 |
93.4 |
30.0 mg 3,5-DCP/L (reference inhibitor) |
4.5 |
97.0 |
* respiration was stimulated, relative to the mean control.
Description of key information
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 393 mg/L
- EC10 or NOEC for microorganisms:
- 500 mg/L
Additional information
The study was conducted using terephthalic acid, the primary degradate of terephthaloyl dichloride, according to OECD 209 under GLP. Activated sludge obtained from a predominantly domestic sewage plant was exposed over a period of three hours to the test substance at nominal concentrations of 500, 1000, 2000 and 4000 mg/L. Inhibition, relative to the mean control rate, occurred in the test substance treatments >1000 mg/L and the 3-h EC50 was 1393 mg/L. The respiration of activated sludge was unaffected by 3-h exposure to terephthalic acid at nominal concentrations of up to 500 mg/L. Therefore, a NOEC of 500 mg/L has been established.
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