Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Long-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

The test was conducted using Chlorhexidine digluconate as test substance. As Chlorhexidine base is the active component, the results can be converted to equivalents of CHD-Base.
The test on reproduction of Daphnia magna was performed according to the OECD Guideline 211. Monitoring of the Chlorhexidine digluconate concentration revealed that the stability of the test substance concentration was > 80% of nominal. Test parameters were mortality of parent daphnia, reproduction, first brood release, intrinsic rates of natural increase, appearance of neonates, condition of parent daphnia, total length and body weight of parent daphnia.
A steep dose-response relationship was found. While at 0.012 mg CHD-Base/L no significant effects were observed, all parent animals died at 0.034 mg CHD-Base/L. The NOEC was found to be 0.012 mg CHD-Base/L for all examined endpoints.
This result demonstrates that Chlorhexidine does not exert any chronic toxicity in D. magna and that solely an acute mode of action can be assumed. This conclusion is in full accordance with the acute D. magna EC50 of 0.049 mg CHD-Base/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
0.012 mg/L

Additional information