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EC number: 225-306-3 | CAS number: 4767-03-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30 August 1993 - 02 September 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study was performed to an OECD test guideline and a statement to GLP compliance is included in the study report.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2,2-bis(hydroxymethyl)propionic acid
- EC Number:
- 225-306-3
- EC Name:
- 2,2-bis(hydroxymethyl)propionic acid
- Cas Number:
- 4767-03-7
- Molecular formula:
- C5H10O4
- IUPAC Name:
- 3-hydroxy-2-(hydroxymethyl)-2-methylpropanoic acid
- Reference substance name:
- Bis-MPA
- IUPAC Name:
- Bis-MPA
- Details on test material:
- - Name of test material (as cited in study report): dimethylolpropionic acid or 2,2-bis(hydroxymethyl)propionic acid (reported as Bis-MPA in this summary)
- Lot/batch No.: 63004-14
Constituent 1
Constituent 2
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
No applicable
Sampling and analysis
- Analytical monitoring:
- not specified
- Details on sampling:
- Not applicable
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- - Source (laboratory, culture collection): Inoculum taken from Toxicon's own culture, which originated from the Swedish Environmental Protection Agency, Stockholm, Sweden.
-clone quoted as SEPA-clone
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- Not reported.
Test conditions
- Hardness:
- Not applicable
- Test temperature:
- 23.5 (start of exposure) to 25.0 ºC (after 72 hours exposure).
- pH:
- pH in the nominal 1000 mg/L treatment was 3.5, the pH was therefore adjusted to 8.3 ± 0.2, adjusted with 1M NaOH. The initial pH in the control was 8.1, in the 100 mg/L treatment 8.4 and the 1000 mg/L 8.3, at the end of the 72 hour exposure period the pH values were 8.8 (control), 9.1 (100 mg/L) and 7.9 mg/L (1000 mg/L.)
- Dissolved oxygen:
- Not applicable
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- 0, 100, and 1000 mg/L.
- Details on test conditions:
- TEST SYSTEM
- Test vessels: 250 mL glass E-bottles with 100 mL solution
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 5
GROWTH MEDIUM
- Standard medium used: not specified
- Detailed composition if non-standard medium was used: refer to further details on methods.
OTHER TEST CONDITIONS
- Adjustment of pH: because of low pH, test solutions were adjusted with 1M NaOH solution to 8.3 ± 0.2.
- Photoperiod: continuous illumination
- Light intensity and quality:100 µE/m2/s
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : sampling at 0, 24, 48, and 72 hours
- Determination of cell concentrations: Fluorescence
- Chlorophyll measurement: Yes - Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 750 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- EC50 was graphically determined to be 750 mg/L.
- Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- Graphical estimation of the EC50
Growth rate calculated as per the OECD guidelines
Any other information on results incl. tables
Algal growth rates and percentage growth rate inhibition relative to the control.
|
Control |
100 mg/L |
1000 mg/L |
Growth rate, µ |
1.83 |
1.84 |
0.58 |
Percent inhibition |
0 |
-0.7 |
68.4 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 72 hour algal growth inhibition study, test species, Selenastrum capricornutum, was performed to determine the toxic effects of the test substance Bis-MPA . The test was conducted according to OECD test guideline 201, and performed to GLP. The 72 hour EC50 value, based on growth rate, was calculated as 750 mg/L (based on nominal exposure concentrations).
- Executive summary:
A 72 hour algal growth inhibition study, test species, Selenastrum capricornutum, was performed to determine the toxic effects of the test substance Bis-MPA. The test was conducted according to OECD test guideline 201, and performed to GLP. The 72 hour EC50 value, based on growth rate, was calculated as 750 mg/L (based on nominal exposure concentrations).
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