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EC number: 225-306-3 | CAS number: 4767-03-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1993-08-30 - 1993-09-02
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Name of the test material used in the study report: 2.2-bis(hydroxymethyl)propionic acid
- Batch no.: 63004-14 - Analytical monitoring:
- yes
- Details on sampling:
- Portions of the stock and test solutions were sampled and delivered to the sponsor for analysis.
- Vehicle:
- no
- Details on test solutions:
- Not applicable
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra fish
ACCLIMATION
- Acclimation period: 14 days in internal quarantine. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- Not applicable
- Hardness:
- 250 ± 25 mg calcium carbonate/L (nominal value)
- Test temperature:
- 24±1ºC
- pH:
- 6.5 - 8.7 (recorded values)
- Dissolved oxygen:
- The dilution water was aerated to oxygen saturation exceeding 90%.
The dissolved oxygen concentration in the test vessels was at least 60% of the air saturation value and ranged between 60 - 100 %. - Salinity:
- Not applicable
- Nominal and measured concentrations:
- 0, 100, and 1000 mg/L (nominal values)
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: not specified
- Material, size, headspace, fill volume: 5 L glass vessels
- Aeration: test solutions were aerated prior to use (>90% oxygen saturation)
- Renewal rate of test solution (frequency/flow rate): test solutions were renewed every 24 hours
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.6 grams of fish per litre
OTHER TEST CONDITIONS
- Adjustment of pH: Not reported, although adjustment (to pH 7.8 ± 0.2) permitted using NaOH or HCl as appropriate.
- Photoperiod: 16 hour light, 8 hours dark.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- No treatment related mortalities occurred during the test period.
- Results with reference substance (positive control):
- The sensitivity of the fish population used for potassium dichromate was 366 mg/L which is in accordance with the Swedish Standard SS 02 81 62
- Reported statistics and error estimates:
- Not applicable
- Validity criteria fulfilled:
- yes
- Conclusions:
- In a short term toxicity test with fish (Danio rerio), no mortalities were observed and the LC50 value over the 96 hour test period was >1000 mg/L (based on nominal exposure concentrations).
- Executive summary:
In a 96-h acute toxicity study, zebrafish (Danio rerio) were exposed to Bis-MPA at nominal concentrations of 0, 100 and 1000 mg/L under semi-static conditions. The 96-h LC50 was >1000 mg/L.
This toxicity study is classified as acceptable and satisfies the guideline requirement for an acute fish toxicity study.
Results Synopsis
Test Type: Static Renewal
LC50: >1000 mg/L
Endpoint Effected: Mortality
Reference
Description of key information
In a short term toxicity test with fish (Danio rerio), no mortalities were observed and the LC50 value over the 96 hour test period was >1000 mg/L (based on nominal exposure concentrations).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- > 1 000 mg/L
Additional information
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