Registration Dossier

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1993-08-30 - 1993-09-02
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
- Name of the test material used in the study report: 2.2-bis(hydroxymethyl)propionic acid
- Batch no.: 63004-14
Analytical monitoring:
yes
Details on sampling:
Portions of the stock and test solutions were sampled and delivered to the sponsor for analysis.
Vehicle:
no
Details on test solutions:
Not applicable
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebra fish

ACCLIMATION
- Acclimation period: 14 days in internal quarantine.

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
Not applicable
Hardness:
250 ± 25 mg calcium carbonate/L (nominal value)
Test temperature:
24±1ºC
pH:
6.5 - 8.7 (recorded values)
Dissolved oxygen:
The dilution water was aerated to oxygen saturation exceeding 90%.
The dissolved oxygen concentration in the test vessels was at least 60% of the air saturation value and ranged between 60 - 100 %.
Salinity:
Not applicable
Nominal and measured concentrations:
0, 100, and 1000 mg/L (nominal values)
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type: not specified
- Material, size, headspace, fill volume: 5 L glass vessels
- Aeration: test solutions were aerated prior to use (>90% oxygen saturation)
- Renewal rate of test solution (frequency/flow rate): test solutions were renewed every 24 hours
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.6 grams of fish per litre


OTHER TEST CONDITIONS
- Adjustment of pH: Not reported, although adjustment (to pH 7.8 ± 0.2) permitted using NaOH or HCl as appropriate.
- Photoperiod: 16 hour light, 8 hours dark.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality.

Reference substance (positive control):
yes
Remarks:
Potassium dichromate
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
No treatment related mortalities occurred during the test period.
Results with reference substance (positive control):
The sensitivity of the fish population used for potassium dichromate was 366 mg/L which is in accordance with the Swedish Standard SS 02 81 62
Reported statistics and error estimates:
Not applicable
Validity criteria fulfilled:
yes
Conclusions:
In a short term toxicity test with fish (Danio rerio), no mortalities were observed and the LC50 value over the 96 hour test period was >1000 mg/L (based on nominal exposure concentrations).
Executive summary:

In a 96-h acute toxicity study, zebrafish (Danio rerio) were exposed to Bis-MPA at nominal concentrations of 0, 100 and 1000 mg/L under semi-static conditions.  The 96-h LC50 was >1000 mg/L.   


This toxicity study is classified as acceptable and satisfies the guideline requirement for an acute fish toxicity study.


 Results Synopsis


Test Type: Static Renewal


LC50:  >1000 mg/L                        


Endpoint Effected: Mortality

Description of key information

In a short term toxicity test with fish (Danio rerio), no mortalities were observed and the LC50 value over the 96 hour test period was >1000 mg/L (based on nominal exposure concentrations).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Dose descriptor:
LC50
Effect concentration:
> 1 000 mg/L

Additional information