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EC number: 225-306-3 | CAS number: 4767-03-7
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30 August 1993 - 02 September 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study was performed according to an appropriate OECD test guideline, and a statement of GLP compliance is included in the report.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2,2-bis(hydroxymethyl)propionic acid
- EC Number:
- 225-306-3
- EC Name:
- 2,2-bis(hydroxymethyl)propionic acid
- Cas Number:
- 4767-03-7
- Molecular formula:
- C5H10O4
- IUPAC Name:
- 3-hydroxy-2-(hydroxymethyl)-2-methylpropanoic acid
- Reference substance name:
- Bis-MPA
- IUPAC Name:
- Bis-MPA
- Details on test material:
- - Name of test material (as cited in study report): dimethylolpropionic acid or 2,2-bis(hydroxymethyl)propionic acid (reported as Bis-MPA in this summary)
- Lot/batch No.: 63004-14
Constituent 1
Constituent 2
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Portions of the stock and test solutions were sampled and delivered to the sponsor for analysis.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Not applicable
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra fish
ACCLIMATION
- Acclimation period: 14 days in internal quarantine.
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- Not applicable
Test conditions
- Hardness:
- 250 ± 25 mg calcium carbonate/L (nominal value)
- Test temperature:
- 24±1ºC (nominal value)
- pH:
- 6.5 - 8.7 (recorded values)
- Dissolved oxygen:
- 60 - >100% (recorded values)
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- 0, 100, and 1000 mg/L (nominal values)
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): not specified
- Material, size, headspace, fill volume: 5 L glass vessels
- Aeration: test solutions were aerated prior to use (>90% oxygen saturation)
- Renewal rate of test solution (frequency/flow rate): test solutions were renewed every 24 hours
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.6 grams of fish per litre
OTHER TEST CONDITIONS
- Adjustment of pH: Not reported, although adjustment (to pH 7.8 ± 0.2) permitted using NaOH or HCl as appropriate.
- Photoperiod: 16 hour light, 8 hours dark.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- No treatment related mortalities occurred during the test period.
- Results with reference substance (positive control):
- The sensitivity of the fish population used for potassium dichromate was 366 mg/L which is in accordance with the Swedish Standard SS 02 81 62
- Reported statistics and error estimates:
- Not applicable
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- An acute semi-static toxicity study in zebra fish (Brachydanio rerio) was performed to assess the toxicity of Bis-MPA. The study was performed to GLP and according to OECD 203 Test Guideline. No mortalities were observed during the test and the LC50 value over the 96 hour test period was observed to be >1000 mg/L (based on nominal exposure concentrations).
- Executive summary:
A 96 hour acute semi-static toxicity study in zebra fish (Brachydanio rerio) was performed to assess the toxicity of Bis-MPA. The study was performed to GLP and according to OECD 203 Test Guideline. No mortalities were observed during the test and the LC50 value over the 96 hour test period was observed to be >1000 mg/L (based on nominal exposure concentrations).
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