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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Test procedure in accordance with German national standards

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report Date:
1989

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: DIN 38412 Teil 9
Principles of method if other than guideline:
Scenedesmus-growth inhibition test
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
Stock solution preparation: a 10 g/L stock solution was prepared in a graduated cylinder using sterile aqua bidest. No additives or solvents were used.

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
Species/strain and source: Scenedesmus subspicatus, breeded at the testing facility

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
20 +/- 1°C
Nominal and measured concentrations:
Nominal concentrations in mg/L: 2, 16, 32, 64, 128, 256, 512
Details on test conditions:
- Growth/test medium chemistry: pH 8.2 +/- 1
- Exposure vessel type: test tubes
- Light levels and quality during exposure: permanent light (10 000 lux, white light, 0.72E20 photones/sec, 120 uE)
- Four replicates per treatment and eight in the control
- Test tubes were shaked twice a day to keep algae in suspension
- Statistical methods: assessment was done by graphical analysis

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC0
Effect conc.:
16 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
85 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
260 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC90
Effect conc.:
700 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate

Any other information on results incl. tables

Was control response satisfactory: yes

Applicant's summary and conclusion