Lecithins, acetylated

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Analytical monitoring:

no

Details on test solutions:

The nominal limit test concentration was prepared by direct addition of the test substance to Haskell well water (HWW). The 100 mg/L nominal limit test solution was prepared by adding approximately 100 mg of test material to HWW in a 1-L glass beaker (final volume 1 litre), and sonicating for approximately 30 minutes and then stirring for approximately 20 minutes. The limit test substance solution was very cloudy with undissolved test material present.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM:
- Strain: Daphnia magna
- Age at study initiation: Neonates,< 24 hours
- Feeding during test: None

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

100-140 mg/L

Test temperature:

18.2-20.2°C

pH:

8.1-8.2

Dissolved oxygen:

6.1-8.3 mg/L

Nominal and measured concentrations:

Nominal limit concentration of 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 mL (nominal) glass beakers containing 200 mL of test solution; The test chambers were covered with a glass plate during the test.
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Conductivity: 325-420 µmhos/cm

OTHER TEST CONDITIONS
- Adjustment of pH: Not reported
- Photoperiod: 16 hour light and 8 hour dark, which included 30 minutes of transitional light (11-25 Lux) preceding and following the 16-hour light interval

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): The criterion for the effect (immobility) was a lack of reaction to application of a gentle stimulus.

TEST CONCENTRATIONS
One nominal limit test concentration and a dilution water control were used in this study. The limit test concentration of 100 mg/L was chosen for the definitive limit test based on the results of a preliminary rangefinding study.

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Results with reference substance (positive control):

No immobility was seen in the dilution water at the end of 48 hours.

Validity criteria fulfilled:

yes

Conclusions:

48-hour EC50 > 100 mg/L

Executive summary:

The acute toxicity of the test substance to unfed Daphnia magna neonates, less than 24 hours old at test start, was determined in an unaerated, static, acute, 48-hour limit test. The test was conducted in accordance with the OECD Guideline for the Testing of Chemicals: 202. The study was conducted with a single 100 mg/L nominal concentration of test substance and a dilution water control at a mean temperature of 19.1°C (range of 18.2-20.2°C). Four replicates with five daphnids per replicate were used for the limit test concentration and four replicates with five daphnids per replicate were used per control. No immobility was seen at the 100 mg/L nominal limit test concentration at the end of the 48-hour limit test. No immobility or sublethal effects were seen in the dilution water control daphnids. The 48-hour EC₅₀, based on the test substance concentration and immobility, was greater than 100 mg/L. 

Description of key information

48-hour EC50> 100 mg/L

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L